潜阳解毒通络方对自发性高血压大鼠心肌纤维化的影响

Qianyang Jiedu Tongluo Decoction on the Role of Myocardial Fibrosis Mechanism in Spontaneously Hypertensive Rats

目的观察潜阳解毒通络方在自发性高血压大鼠(SHR)心肌组织胶原合成和心肌纤维化中的作用。方法 12周龄雄性自发性高血压大鼠(SHR)分为SHR应用潜阳解毒通络方组(QYTL组)、SHR应用糜酶抑制剂组(Chy-I组)、SHR应用潜阳解毒通络方及糜酶抑制剂组(QY&Chy-I组)、SHR组,另设10只12周龄的雄性Wistar-Kyoto大鼠(WKY)为正常对照组。检测各组大鼠的收缩压、心肌胶原容积分数(CVF)、心肌血管周围胶原面积比(PVCA)和心肌糜酶及I、III型胶原m RNA表达水平。结果QYTL组、QY&Chy-I组和Chy-I组心脏CVF、PVCA与SHR组比较显著下降(P<0.01),与WKY组比较无显著差异。QYTL组、QY&Chy-I组和Chy-I组心肌组织I、III型胶原和糜酶m RNA表达相对含量均低于SHR组(P<0.01),与WKY组比较无显著性差异。QYTL组、QY&Chy-I组大鼠血压与SHR比较有显著改变,Chy-I组大鼠血压与SHR比较无显著改变。结论心肌组织糜酶参与胶原的合成及细胞外基质的形成和降解,潜阳解毒通络方可能通过抑制糜酶途径改善心肌纤维化。

Objective Research of Qianyang Jiedu Tongluo（QYTL）decoction inhibition of Myocardial tissue collagen synthesis and myocardial fibrosis to explore its mechanism in spontaneously hypertensive rats.Methods Through using the method of pathology,computer analysis and reverse transcription polymerase chain reaction,the spontaneously hypertensive rats divided into QYTL group,Chy-I group,the QY Chy-I group,SHR group,WKY group.Testing the systolic pressure,CVF,PVCA,the myocardial chymase and collagen I and III mRNA expression.Results Compared with the WKY group,CVF and PVCA mRNA expression was significantly inhibited（P〈0.01） in QYTL group,Chy-I group,the QY Chy-I group.Compared with the SHR group,the myocardial chymase and collagen I and III mRNA expression was significantly inhibited（P〈0.01） in QYTL group,Chy-I group,the QY Chy-I group.Compared with the SHR group,the systolic pressure was significantly inhibited（P〈0.01） in QYTL group and the QYChy-I group.Conclusion The myocardial chymase involved in the synthesis of collagen,the formation and degradation of ECM,and promote myocardial fibrosis in spontaneously hypertensive rats,while QYTL could Inhibition myocardial fibrosis through the way of Inhibiting the myocardial chymase.