行为学评价大鼠急性脊髓损伤模型脊髓节段选择的研究

Study of Spinal Cord Segmental Choice after Spinal Cord Injury in Rats With Behavior Score

目的通过行为学评价观察不同脊髓损伤节段选择的特点及对脊髓功能恢复的影响。方法选取不同脊髓损伤节段建立大鼠急性脊髓损伤模型。BBB评分法和斜板试验观察1d、3d、5d、7d、14d、21d和28d脊髓功能恢复情况。结果通过BBB评分法和斜板试验观察显示,脊髓损伤组(T8、T9、T10)大鼠相比假手术组大鼠各时间点均有显著差异(P<0.01),整体趋势随时间呈现跳跃式变化。BBB评分结果显示,第5d开始,T10组的评分值高于T8组(P<0.05),第7d到21d,T10组的评分值明显高于T8组(P<0.01),第14d和21d,T10组的评分值高于T9组(P<0.05),在第28d,T10组评分相比T8组和T9组无统计学差异(P>0.05)。斜板实验结果显示,第5d到28d,T10组的评分值高于T8组(P<0.05)。第5d到14d,T10组评分值高于T9组(P<0.05),而第21d和28d,T10组的评分值相比T9组无统计学差异(P>0.05)。评分值标准化为百分制后进行横向比较,假手术组大鼠两种评分值之间的一致性较好,脊髓损伤组大鼠两种评分值之间的差异较大,在术后1d、3d、28d时,无统计学差异(P>0.05),在第7d、14d可观察到差异非常显著(P<0.01)。结论通过BBB评分和斜板试验发现,T10组实验结果所反映的组间差异在术后各时间点较T8、T9差异更加显著,跨度更大,在实验过程中更有利于观察其变化,在损伤后第5d到第21d尤为明显,建立脊髓损伤模型选择T10损伤节段要优于T8和T9损伤节段。

Objective To observe characteristic of spinal cord segmental choice after spinal cord injury in rats with behavior score and the impact of spinal cord function will recover. Methods Select different section of spinal cord injury after model of acute spinal cord injury in rats,to observe Spinal cord function recovery at 1d,3d,5d,7d,14 d,21d and 28 d with BBB scale and inclined plane test. Results There was significant difference between injury groups at all observing points after spinal cord injury（ SCI） compare with sham operation（ P〈0. 01）. BBB scale show values from T10 higher than T8（ P〈0. 05） from 5th day,from 7th day to 21 st day,there was significant difference between T10 and T8（ P〈0. 01）,at 14 th day and 21 th day,values from T10 higher than T9（ P〈0. 05）,this difference was not statistically significant at 28 th day（ P〉0. 05）. Inclined plane test show values from T10 higher than T8（ P〈0. 05） from 5th day to 28 th day,values from T10 higher than T9（ P〈0. 05） from 5th day to 14 th day,this difference was not statistically significant at 14 th day and21 th day（ P〉0. 05）. Horizontal comparison after changed into percentage,values from two evaluation systems in the sham operation group conformed considerably to each other,There was differences between two scales in the spinal cord injury group,This difference was not statistically significant at 1d,3d,28 d after SCI（ P〉0. 05）,however,there was significant difference at 7d and 14d（ P〈0. 01）. Conclusion Through the observation found that T10 group of experimental results reflect that differences between groups at each point in time was more significant and span,more conducive to observe its change in the experimental process,there was the obvious result from 5th day to 21 th day after injury,they are generally that T10 discrimination is superior to T8 and T9.