摘要
SP(Side Population)细胞又叫侧群细胞,是一群能将DNA结合染料Hoechst33342等排出胞外,通过流式细胞仪检测呈Hoechst33342低染的细胞。通过对相关研究分析发现实体肿瘤组织中的SP细胞同样可以通过流式细胞仪分离纯化,由于其具有自我更新、分化等特性被认为其中富集了肿瘤干细胞。目前,在各种实体瘤中都验证了SP细胞的存在,由于具有干细胞及外排内外源化学物质的特性,SP细胞可能是肿瘤复发和耐药的根源,对SP细胞的研究将会给肿瘤的根除提供一套新的策略。
Side population cells is a group of cells with the capacity of efflux DNA fluorescent dye Hoechst 33342 and present a low staining intensity in flow cytometry plot. By analyzing the related research,it＇s found that SP cells in solid tumor tissues can be separated and purified by flow cytometry. Because of the characteristics of self-renewal and differentiation,it is believed that tumor stem cells are enriched. At present,the existence of SP cells in various solid tumors has been verified,because of the characteristics of stem cells and SP cells,SP cells may be the source of tumor recurrence and drug resistance. And the study of SP cells will be provided to tumor eradication of a new strategy.
出处
《世界科技研究与发展》
CSCD
2015年第5期622-626,共5页
World Sci-Tech R&D
关键词
实体瘤
SP细胞
肿瘤干细胞
耐药
solid tumor
side population cell
cancer stem cell
drug-risistance
