结肠癌转移相关基因1在结直肠癌中的表达及与临床病理特征的关系

Expression of MACC1 protein in colorectal cancer and its relationship with clinicopathological characteristics

目的探讨结肠癌转移相关基因1（MACC1）蛋白在结直肠癌组织中的表达及其临床意义。方法采用免疫组织化学方法（免疫组化）检测96例结直肠癌组织及癌旁5cm以上结直肠黏膜组织中MACCl蛋白的表达情况，并分析其与结直肠癌患者临床病理特征之间的关系。结果结直肠癌组织中MACCl蛋白高表达率为75．0％（72／96），显著高于癌旁5cm以上结直肠黏膜组织的14．6％（14／96）（χ2=68．43，P〈0．01）；MACC1的表达与结直肠癌患者的远处转移和TNM分期有关（均P〈0．01），而与患者年龄、性别、肿瘤大小、分化程度、浸润深度以及淋巴结转移等无关（均P〉0．05）。TNM分期越晚，癌组织MACC1的阳性表达率越高；当出现远处转移时，肿瘤组织均高表达MACC1。中位随访13（4～21）月，7例患者死亡，其中MACC1高表达6例（8.3％，6／72），MACC1低表达1例（4．2％，1／24）；9例出现远处转移，其中高表达7例（9．7％），低表达2例（8．3％）；2例局部复发均为高表达。结论MACC1蛋白在结直肠癌组织中高表达，其高表达可能与结直肠癌的侵袭和转移密切相关。

Objective To investigate the expression of metastasis-associated colon cancer 1 （MACC1） protein in colorectal cancer and its clinical significance. Methods Immunohistochemistry method was used to determine the expression of MACC1 protein in colorectal cancer and normal colorectal mucosal tissues （〉5 cm distance to cancer tissue）. Statistic analysis was performed to investigate the association between clinicopathologic features and MACC1 expression. Results The positive rate of MACC1 protein in colorectal cancer tissues was significantly higher than that in normal tissues [75%（72/ 96） vs. 14.6% （14/96）, P〈0.01, χ2=68.43]. Expression of MACC1 protein was associated with TNM staging（P〈0.01, χ2=16.82）and distant metastasis （P〈0.01, χ2=10.53）, but not with age, gender, tumor size, differentiation degree, invasion depth, and lymph node metastasis （all P〉0.05）. Positive rate of MACC1 expression increased with the advanced TNM staging. When distant metastasis occurred, high expression of MACC1 protein in cancer tissues was found. During median 13（4 to 21） months of follow-up, 7 patients died, including 6（8.3%, 6/72） with high expression and 1 （4.2%, 1/24） with low expression. Distant metastasis occurred in 9 patients, including 7 with high expression and 2 with low expression. Two patients had local relapse, whose MACC1 expressions were both high.Conclusion MACC1 protein is highly expressed in colorectal cancer tissues, which may be associated with the invasion and metastasis of colorectal cancer.