被动免疫治疗过敏性鼻炎豚鼠对炎症细胞因子的影响

Influence on inflammation cytokines in passive immunotherapy for allergic rhinitis guinea pigs

目的：探讨抗IL－1β和TNF－αIgY滴鼻治疗过敏性鼻炎豚鼠对炎症细胞因子的影响。方法：应用卵清白蛋白（ OVA）建立豚鼠过敏性鼻炎模型。将豚鼠随机分为：正常对照组（ C组，17只），模型组（ M组，27只），0．1％抗IL－1β和TNF－αIgY治疗组（ Z1组，21只），丙酸氟替卡松治疗组（ Z2组，21只）。分别在治疗后2、4、8 h心脏取血，鼻灌洗收集鼻灌洗液（ NLF），ELISA法测定细胞因子水平。结果：在外周血，0．1％抗IL－1β和TNF－αIgY治疗组2～8 h时IL－1β、IL－5、IL－9、IL－13、IL－18、IL－33和TGF－β1，2～4 h时TNF－α和OVA特异性IgE，以及4～8 h时IL－22等水平均显著低于模型组（P＜0．05）。在鼻灌洗液中，0．1％抗IL－1β和TNF－αIgY治疗组2～8 h时IL－1β、IL－5、IL－9、IL－13、IL－22、IL－33、TNF－α、TGF－β1和OVA特异性IgE，以及2 h时IL－18等水平显著低于模型组（ P＜0．05）。结论：过敏性鼻炎豚鼠采用抗IL－1β和TNF－αIgY滴鼻治疗能显著地降低炎症因子水平。

Objective：To explore the influence on inflammation cytokines for anti-IL-1βand TNF-αIgY intranasal treatment in guinea pigs with allergic rhinitis.Methods：The allergic rhinitis model in guinea pigs was established using ovalbumin（OVA）.Hartley guinea pigs were randomly divided into the control group（group C,n=17）,the allergic rhinitis model group（group M,n=27）,the 0.1%anti-IL-1βand TNF-αIgY treatment group（group Z1,n=21）and the fluticasone propionate treatment group（group Z2,n=21）. At 2 h,4 h and 8 h after the last treatment,blood was got by heart puncture,as well as nose was lavaged using 0.9% saline and the nasal lavage fluid（ NLF） was collected.The level of cytokines was examined using ELISA kits.Results： In the peripheral blood, the levels of IL-1β,IL-5,IL-9,IL-13,IL-18,IL-33 and TGF-β1 from 2 h to 8 h;TNF-αand OVA-specific IgE from 2 h to 4 h;and IL-22 from 4 h to 8 h were significantly decreased in the 0.1%anti-IL-1βand TNF-αIgY treatment group compared with the allergic rhinitis model group（P〈0.05）.In the NLF,the levels of IL-1β,IL-5,IL-9,IL-13,IL-22,IL-33,TNF-α,TGF-β1 and OVA-specific IgE from 2 h to 8 h;and IL-18 at 2 h were significantly decreased in the 0.1% anti-IL-1βand TNF-αIgY treatment group compared with the allergic rhinitis model group （ P〈0.05 ） .Conclusion： Anti-IL-1βand TNF-αIgY intranasal treatment can significantly reduce inflammation cytokine levels in allergic rhinitis guinea pigs.