抑制URG4表达对宫颈癌小鼠的治疗作用及机制

Analyze curative effect of suppression expression of URG4 on cervical cancer mice and possible mechanisms

目的:分析上调基因-4(URG4)在宫颈癌中的表达,并观察分析抑制URG4对宫颈癌的治疗作用及机制。方法:Western blot法分析URG4在宫颈癌组织及癌旁组织中的表达差异。选择16只雄性BALB/c裸鼠,喂养至6周龄后随机分为对照组(Control组)及观察组(Observation组),每组各8只。Control组小鼠于右侧背部皮下接种宫颈癌细胞(Si Ha cells)。Observation组小鼠背部皮下接种URG4-si RNA转染的宫颈癌细胞(URG4-si RNA Si Ha cells)。干预后4周处死小鼠,分析对比小鼠肿瘤生长情况及Wnt/β-catenin信号通路的改变。结果:URG4在人宫颈癌组织中呈现高表达,显著高于癌旁组织(P<0.05)。干预结束后,观察组小鼠肿瘤组织内URG4表达量显著低于对照组(P<0.05);体重明显高于对照组(P<0.05);肿瘤组织重量显著低于对照组(P<0.05)。观察组小鼠肿瘤组织内Ki67表达量显著低于对照组(P<0.05)。观察组小鼠肿瘤组织内Cyclin D1、β-catenin及C-myc的表达均显著低于对照组,相比较差异均具有统计学意义(P<0.05)。结论:URG4在人宫颈癌中呈现高表达,抑制URG4的表达可能通过抑制Wnt/β-catenin信号通路而发挥抗肿瘤效应。

Objective：To analyze the curative effect of suppression expression of URG4 on cervical cancer mice and the possible mechanisms.Methods：The expression of URG4 were analyzed in cervical cancer tissues and para-carcinoma tissues.16 male BALB/c nude mice were divided into Group A （ inoculated with URG4-siRNA SiHa cells） and Group B （ inoculated with SiHa cells） . After 4 weeks,mice were executed and analyzed.Results：The expression of URG4 were higher in human cervical cancer tissues than that in para-carcinoma tissues （ P〈0.05）.After experiment,Group A had a lower tumor weight,but a higher body weight than these of Group B,the difference was statistically significant （P〈0.05）.Group A mice had a lower levels of URG4 in tumor than that of Group B mice （P〈0.05）.Group A mice had a lower levels of Ki67 in tumor than that of Group B mice,the difference was statistically significant （P〈0.05）.Group A mice had lower levels of CyclinD1,β-catenin and C-myc in tumor than these of Group B mice （P〈0.05）. Conclusion：The expression of URG4 in cervical cancer tissue is up-regulation.Suppression expression of URG4 can suppress tumor growth in vivo,which may though suppress the Wnt/β-catenin signaling pathway.