黄芩中抗菌有效成分提取工艺研究

Extraction of Effective Ingredients from Scutellaria Bacalensis Georgi

在单因素试验的基础上,选取提取温度、提取时间和液料比为影响因素,应用Box-Behnken中心组合进行3因素3水平的试验设计,以对白色念珠菌产生的抑菌圈直径作为响应值,对结果进行多元线性和二项式拟合,采用响应面(RSA)法选取黄芩中抗菌有效成分提取的最佳工艺条件。最佳工艺条件为:提取温度61℃,提取时间2 h,液料比13∶1(m L∶g),经3次验证实验得黄芩水提物质量浓度为0.5 g/m L时,抑菌圈平均直径为12.637 mm,与预测值12.665 mm接近,阳性对照枯草杆菌肽的平均抑菌圈直径为11.85 mm,表明响应面法优化得到的黄岑水提物对白色念珠菌的抑菌作用显著。

In order to optimize the extraction condition for effective active ingredient from Scutellaria Bacalensis Georgi, a three- factor-three-level experiments design was developed by using Box-Benhken central composite design method based on single-factor experiments with extraction temperature, extraction time, solvent-solid ratio as influence factors. The bacteriostatic ring diameter against Candida albicans was used as the responsive values. The data were fitted by using multi-linear equation and second-order polynomial equation. And the extraction conditions were optimized by response surface method. The optimum conditions were as follows： extraction temperature 61 ℃, extraction time 2 h and solvent-solid ratio 13： 1. Under these conditions, the average diameter of bacteriostatic circles verified by three experiments was 12. 637 mm closing to the predicted value（ 12. 664 88 mm）, when the concentration of extractions was 0.5 g/mL. And the average diameter of bacteriostatic circle of positive contrast was 11.85 mm. The results showed that the aqueous extract of Scutellaria bacalensis Georgi had significant bacteriostatic effects on Candida albicans.