土槿皮等5种中药及其不同组合抗糠秕马拉色菌的体外药敏试验研究

Inhibitive effect of five kinds of Chinese herbal and their differentcombination on Malassezia furfur in vitro

目的测定土槿皮、侧柏叶、苦参、丁香、肉桂及其不同组合对糠秕马拉色菌的最小抑菌浓度,观察其体外抑菌效果。方法参照美国国家临床实验室标准化委员会制订的M27-A方案中酵母菌微量稀释法,在此基础上进行改良,采用含油的培养基作为药敏试验的液基,制备微量药敏板,对糠秕马拉色菌进行体外药敏试验。结果最低抑菌浓度(minimum inhibitory concentration,MIC)依次为:5种中药混合液(槿柏洗剂):5.5 mg/m L,土槿皮+侧柏叶+苦参+丁香:16 mg/m L,土槿皮+侧柏叶+苦参:8.5 mg/m L,丁香+肉桂:9 mg/m L,土槿皮:10 mg/m L。结论相比于各单味药及不同组合,槿柏洗剂全方MIC为最小,5种中药及其不同组合混合液均有抑制糠秕马拉色菌生长的作用。

Objective To observe the inhibitive effect of the cortex pseudolaricis, oriental arborvitae, sophora flavescens ait, clove, cinnamon and their different combination on Malassezia furfur in vitro. Methods Based on the M27-A scheme about yeast micro dilution method, which was developed by the national clinical laboratory standardization committee （NCCLS）,we prepared drug sensitive plate and liquid medium with oil to test the sensitivity of Chinese herb to Malassezia furfur. Results Minimum inhibitory concentration（MIC）：5 kinds of Chinese herbal mixture （the Jin Bai lotion）：5. 5mg/mL,cortex pseudolaricis＋oriental arborvitae＋sophora flavescens ait＋clove：16mg/mL, cortex pseudolaricis＋oriental arborvitae＋sophora flavescens ait：8. 5mg/mL, clove＋cinnamon：9mg/mL, cortex pseudolaricis：10mg/mL. Conclusions Compared to the single herb and the different combinations, the MIC of Jin Bai lotion was the smallest. Five kinds of Chinese herbal and their different mixture can inhibit the Malassezia furfur.