元胡总碱对实验性大鼠胃黏膜损伤的保护作用

Protective effect of total alkaloids of Rhizoma Corydalis on experimental gastric mucosal lesions in rats

目的研究元胡总碱(TARC)对实验性大鼠胃黏膜损伤的保护作用。方法利用胃黏膜下注射冰乙酸方法制备大鼠急性胃黏膜损伤模型。第2天,按照分组分别ig给予西咪替丁400 mg·kg-1,TARC 20,40和80 mg·kg-1,连续ig 15 d,末次给药后2 d,测定大鼠胃液分泌量及胃液总酸度;HE染色观察胃组织病理变化,计算胃黏膜损伤指数和损伤抑制率;免疫组织化学法测定胃黏膜表皮生长因子(EGF)和EGF受体(EGFR)的表达水平。结果与假手术组比较,模型组大鼠胃黏膜损伤面积和损伤指数显著增加(P<0.01),提示大鼠胃黏膜损伤模型制备成功。与模型组比较,西咪替丁组和TARC 80 mg·kg-1组分别降低胃黏膜损伤面积的39.9%和23.7%,胃黏膜损伤指数分别降低52.3%和30.5%(P<0.01),TARC 80 mg·kg-1组与西咪替丁组比较无统计学差异。与模型组比较,西咪替丁组及TARC 40和80 mg·kg-1组大鼠胃黏膜EGF蛋白表达分别增加81.8%,24.2%和57.6%,EGFR蛋白表达分别增加45.9,16.2%和29.7%。病理学观察发现,模型组胃黏膜脱落坏死,大量炎性细胞浸润;西咪替丁组、TARC 40和80 mg·kg-1组胃组织病变明显改善。结论 TARC具有保护大鼠急性胃黏膜损伤的作用,其机制可能与上调胃黏膜组织中EGF和EGFR的表达有关。

OBJECTIVE To investigate the protective effect of total alkaloids of Rhizoma Corydalis（TARC） on experimental gastric mucosal lesions in rats. METHODS Gastric mucosal lesions were induced in rats by injecting acetic acid under gastric mucosal. From the 2nd day post the preparation of the rat model, cimetidine 400 mg·kg- 1or TARC 20, 40 and 80 mg·kg- 1was ig delivered for 15 d in different groups. Two days after the last delivery, gastric juice volume and total acidity were measured.Histopathology of stomach tissues was observed by HE staining. The area of gastric ulcer area was measured and the ulcer index and ulcer inhibitory rate were calculated. The expression of epidermal growth factor（EGF） and EGF receptor（EGFR） was detected by immunohistochemical staining.RESULTS Comparing with shame group, the eare of gastric ulcer and ulcer index were increased significantly in the model group（P〈0.01）, suggesting that the model rats were prepared properly. Compared with the model group, the ulcer area in rats of cimetidine and TARC 80 mg·kg-1groups was decreased by 39.9% and 23.7%,respectively. The ulcer index was decreased by 52.3% and 30.5%,respectively.There was no significant difference between the cimetidine group and TARC 80 mg·kg- 1group, in the ulcer area or index. Compared with model group, EGF protein expression of cimetidine and TARC 40 and 80 mg·kg-1groups was increased by 81.8%,24.2% and 57.6%,respectively while EGFR protein expression was increased by 45.9%,16.2% and 29.7%,respectively（P〈0.05,P〈0.01）. Deciduous and necrotic gastric mucosal and a large amount of inflmmatory cells infiltration were observed in model group, and gastric mucosal lesions were improved in cimetidine and TARC 40 and 80 mg·kg- 1groups.CONCLUSION TARC has protective effect on gastric mucosal lesions in rats. The mechanism may be related to the increase of EGF and EGFR protein expression.