正交设计优化当归多糖的定量分析方法

Optimal Quantitative Analysis Method for Polysaccharide in Angelica Sinensis with Orthogonal Design

目的优化当归多糖苯酚-硫酸显色法的测定条件。方法采用苯酚-硫酸显色法,以对照品葡萄糖的含量为考察指标,检测波长为483 nm,按L9（34）正交试验设计筛选最佳显色条件。结果浓硫酸和苯酚用量对多糖含量测定的影响较显著,优选出的多糖最佳显色条件为苯酚1.5 ml、浓硫酸7 ml、反应温度100℃、反应时间为10 min,线性范围为0.005~0.02 mg/ml,平均回收率为101.3%,相对标准偏差为1.22%。结论苯酚硫酸法快速、简便,准确性高、重复性好,可适用于当归多糖的含量测定。

Objective To optimize the detection condition of coloration method with phenol- sulphuric acid for polysaccharide in Angelica Sinensis. Methods The glucose content of reference substance was used as a parameter,and the coloration method with phenol- sulphuric acid was used to detect the best coloration method with 483 nm wave length and optimized with the L9（ 34） orthogonal design. Results The concentrated sulfuric acid and phenol volumes had significant effects on detecting polysaccharide content. The best coloration method for polysaccharide was 1. 5 ml phenol,7 ml concentrated sulfuric acid,100℃ reactive temperature,10 min reactive time,0. 005-0. 02 mg / m L linear range,101. 33%average recovery and 1. 22% RSD（ relative standard deviation）. Conclusion The phenolsulphuric is rapid,simple and accurate with good repeatability,and can been applied in content detection of polysaccharide in Angelica Sinensis.