大肠杆菌araE基因对典型苯丙素类化合物转运过程的影响

Identification of ara E Gene Associated with Response to Different Phenylpropanoids by Gene Silencing in Escherichia coli BL21

为了研究阿拉伯糖转运蛋白对大肠杆菌转运典型苯丙类素化合物的能力,采用基因过量表达和基因沉默技术,实现对蛋白质表达量进行控制,从而研究Ara E对苯丙类素化合物的转运过程机制。同时,用定量PCR技术在转录水平考察并验证了ara E基因的过量表达水平和基因抑制程度。通过测定菌体的生长动力学参数,衡量菌体对典型苯丙素类化合物的转运能力。结果表明,大肠杆菌ara E基因只参与了大肠杆菌对白藜芦醇的转运,对其他种类的苯丙素类化合物作用不明显。

Ara E is an important unidirectional transport channel protein that could transport some compounds outside the cell. E. coli is a common host-bacterial to produce phenylpropanoids,and the growth was inihibited by phenylpropanoids. In order to investigate the transport ability of Ara E on phenylpropanoids in E. coli,the gene overexpression and silencing routes was used in this study to regulate the expression of Ara E. The level of overexpression and silencing was confirmed by q PCR and SDS-PAGE. The transport ability of phenylpropanoids was measured by detection of the recombinant bacterial growth kinetics parameter（μmax） in different typical phenylpropanoids（rutin,naringenin and resveratrol）. The result showed that Ara E could only transport resveratrol in E. coli.