金黄色葡萄球菌标准物质的定值方法

Discussion About the Fixed Value Method of Staphylococcus aureus Reference Materials

对含鸡肉基质的金黄色葡萄球菌标准物质进行定值,包括金黄色葡萄球菌计数方式的选择,标准值的测定,以及不确定度的分析。总不确定度以扩展不确定度表示。结果显示,金黄色葡萄球菌3种计数方式,BP平板倾注法计数结果与PCA计数法测定一致,BP平板倾注法SD值<BP平板涂布法SD值,表明采用BP平板倾注法计数金黄色葡萄球菌的结果精密度、再现性较好,有利于试验结果的复现。标准值测定结果为275 CFU/瓶;总不确定度为6。因此,标准物质定值结果为(275±6)CFU/瓶。

To quantify the SD value of Staphylococcus aureus Reference Materials in chicken matrix,the S.aureus counting methods,the standard values and the uncertainty were analyzed in this study. As compared to the results of three different S. aureus counting methods,BP pouring counting method showed the same result with PCA counting method,The SD value of BP tablet pouring method was smaller as compared to BP tablet coating method.The BP tablet pouring counting showed good precision and reproducibility. The SD values was 275 CFU/bottles and the total uncertainty was 6,the Reference Materials constant value was（275 ＋ 6） CFU/bottle.