eIF4E、VEGF-A和VEGF-C在胃癌组织中的表达及其与淋巴结侵袭转移的相关性

The expression of eIF4E,VEGF-A and VEGF-C in gastric carcinoma tissues and their correlation with lymph node invasion and metastasis

目的通过检测真核细胞起始因子4E(eIF4E)、血管内皮生长因子(VEGF)-A和VEGF-C蛋白在胃癌组织中的表达,探讨它们在肿瘤侵袭和淋巴结转移过程中的作用。方法采用免疫组织化学SP法检测58例胃癌组织和25例正常胃黏膜组织中eIF4E、VEGF-A和VEGF-C蛋白的表达。结果 eIF4E、VEGF-A和VEGF-C在胃癌组织中蛋白表达阳性率分别为89.7%(52/58)、65.5%(38/58)、60.3%(35/58),明显高于它们在正常胃黏膜组织中的阳性表达率4.0%(1/25)、12.0%(3/25)、8.0%(2/25),差异均有统计学意义(P<0.05)。eIF4E、VEGF-A和VEGF-C的阳性表达率与患者的年龄、性别均无明显相关性(P>0.05),而与肿瘤的浸润深度、淋巴转移及临床分期有关(P<0.05)。eIF4E和VEGF-C还与肿瘤分化程度有关。胃癌中elF4E的阳性表达与VEGF-A和VEGF-C均呈正相关。结论 eIF4E在胃癌的发生、发展中起关键调控作用,VEGF-A和VEGF-C有助于肿瘤浸润和淋巴结转移。联合检测有助于对胃癌恶性程度的判定和评估。

Objective To investigate the expression of eukaryotic initiation factor 4E（eIF4E） ,vascular endothelial growth factor （VEGF）‐A and VEGF‐C in gastric cancer tissues and their correlation with invasion and metastasis of gastric carcinoma .Methods The expressions of eIF4E ,VEGF‐A and VEGF‐C were detected in tissues of 58 gastric carcinomas and 25 normal gastric mucosa by using immunohistochemical method .Results The positive rate of eIF4E、VEGF‐A and VEGF‐C protein expression were 89 .7%（52/58） ,65 .5% （38/58） ,60 .3% （35/58） in gastric carcinoma ,which were higher than those in normal gastric mucosa tissues which were 4 .0% （1/25） ,12 .0% （3/25） ,8 .0% （2/25） respectively .Expressions of eIF4E ,VEGF‐A and VEGF‐C were significantly correlated with the depth of invasion and lymph node metastasis（P〈0 .05） ,but not with the age ,sex of patients（P〉0 .05） .Ex‐pressions of eIF4E and VEGF‐C were significantly correlated with tumor differentiation .The expression of eIF4E was being found positively correlated with VEGF‐A and VEGF‐C .Conclusion eIF4E may play certain roles in the oncogenesis and progression of gastric carcinoma .VEGF‐A and VEGF‐C may be helpful in lymph metastasis .Combined detection of eIF4E ,VEGF‐A and VEGF‐C may be helpful to assess the malignant degree and prognosis of gastric carcinoma .