滤纸干血斑制备疟原虫DNA模板的方法评价

Method evaluation of plasmodium DNA template extraction from dried blood on filter paper

目的评价滤纸干血斑制备疟原虫DNA模板的方法。方法采用常规DNA抽提程序和PCR扩增程序,收集并检测47份疑似输入性疟疾患者标本,统计分析配对设计的检测结果。结果滤纸干血斑PCR与厚薄血涂片检测结果比较Kappa=0.492,与全血标本PCR检测结果比较Kappa=0.686;分别以厚薄血片镜检和全血标本进行的PCR检测为金标准,滤纸干血斑采集血样进行PCR检测的灵敏度分别为75.0%和71.4%,特异度分别为78.9%和100.0%。结论滤纸干血斑采集血液标本进行PCR检测尚不能取得良好的效果,仍需进一步研究。

Objective To evaluate the method of plasmodium DNA template extraction from dried blood on filter paper . Methods Collectedand tested 47 blood samples of suspected imported malaria patients ,the DNA was extracted and tested by using conventional procedures and PCR ,then count and analyze test results of matched design .Results The Kappa value was 0 .492 when compared filter paper dried blood spots with thick and thin blood smear test results ,and the Kappa value was 0 .686 when com‐pared with whole blood sample .Using the results of thick and thin blood smear and whole blood samples as the gold standard to e‐valuate the sensitivity and specificity of filter paper dried blood spot method respectively ,the sensitivity was 75 .0% ,71 .4% and specificity was 78 .9% ,100 .0% .Conclusion Collected blood samples using filter paper can′t get good results in PCR test ,still needs further research .