响应曲面法优化超声波提取白蒿总黄酮研究

Ultrasonic Extraction of Flavonoids from Herb of Sievers Wormwood and its Optimization by Response Surface Method

在单因素试验的基础上,利用曲面响应法对超声波辅助纤维素酶提取吕梁白蒿总黄酮提取工艺参数进行优化研究。首先利用正交试验优化纤维素酶解条件,然后选择超声波功率、超声波时间和超声波次数为自变量,总黄酮得率为响应值,采用Box-Behnken设计、Design Expert 7.0分析和二次回归多项式预测策略优化超声波条件。结果表明,超声波辅助纤维素酶提取白蒿总黄酮最佳工艺条件为超声波时间14 min,超声波提取3次,超声波功率396 W,酶解时间120 min,加酶量2.5%,酶解温度50℃,酶解p H值5.5,其中超声波时间与次数的交互作用较强。白蒿总黄酮得率理论值为2.43%,实际值为2.47%。此方法优化吕梁白蒿中总黄酮提取效果良好。

In this study, the response surface method on the base of single factor test was used to optimize the extract process of ultrasonic-assisted cellulase extraction of flavonoids from Luliang herb of sievers wormwood. After optimizing cellulase factors by the orthogonal experiment, the ultrasonic factors were studied with Box-Behnken design and polynomial quadratic equation predicting by Expert 7.0 analysis. The best solution was as follows： ultrasonic power 396 W, ultrasonic time 14 min, extracting 3times, 2.5% adding amount of enzyme, enzymolysis time of 120 min at 50 ℃ and p H 5.5. Under the optimized condition, the predicted and actual extraction rate of flavonoids were respectively 2.43% and 2.47%. Thereby the fitted multiple regression equation was credible.