染汞后草鱼组织器官磷酸酶活性的变化

Change of phosphatases activity in different tissues of grass carp(Ctenopharyngodon idella)after exposing to Hg^(2+)

将试验鱼暴露在汞离子浓度为0.5 mg·L-1水体中,24 h后置于正常养殖用水中恢复饲养1,5,12,21 d后分别取样,测定血浆、鳃、肝胰脏、脾脏和肾脏中碱性磷酸酶(Alkaline Phosphatase,AKP)和酸性磷酸酶(Acid Phosphatase,ACP)的活性变化。结果显示,与非染汞对照组相比较,处理组AKP活性变化为:血浆(1 d)、鳃(1,5,12,21 d)、肝胰脏(21 d)和脾脏(1 d)显著下降(P<0.05,P<0.01);肝胰脏(12 d)和肾脏(1 d)显著升高(P<0.01);血浆(5,12,21 d)、肝胰脏(1,5 d)、脾脏(5,12,21 d)和肾脏(5,12,21 d)无显著变化(P>0.05)。ACP活性变化为:鳃(1,5,12,21 d)、肝胰脏(12,21 d)显著降低(P<0.05,P<0.01);血浆(1,5 d)、肝胰脏(1,5 d)、脾脏(1,5 d)、肾脏(1 d)显著升高(P<0.05,P<0.01);血浆(12,21 d)、脾脏(12,21 d)和肾脏(5,12,21 d)无显著变化(P>0.05)。鳃和肝胰脏受Hg2+的影响较血浆、脾脏和肾脏大,其AKP,ACP的活性在试验期内未得到恢复。

In this study,the experimental grass carps were placed in Hg2 ＋（ 0. 5mg·L- 1） for 24 h first and then in cultivated water. Afterwards,they were sampled at different recovering times（ 1,5,12,21 d） to test the activity changes of the alkaline phosphates（ AKP） and acid phosphates（ ACP） in their plasma,gill,hepatopancreas,spleen and kidney. The results were as follows： compared with the control group,the AKP activity in plasma（ 1 d）,gill（ 1,5,12,21 d）,hepatopancreas（ 21 d） and spleen（ 1 d） decreased significantly（ P〈0. 05,P〈0. 01） and increased remarkably in hepatopancreas（ 12 d） and kidney（ 1 d）（ P〈0. 05,P〈0. 01）,while there was no significant differences in plasma（ 5,12,21 d）,hepatopancreas（ 1,5 d）,spleen（ 5,12,21 d） and kidney（ 5,12,21d）（ P〉0. 05）. The ACP activity in gill（ 1,5,12,21 d） and hepatopancreas（ 12,21 d）,spleen（ 1,5 d） dropped remarkably（ P〈0. 05,P〈0. 01） and rose remarkably in plasma（ 1,5 d）,hepatopancreas（ 1,5 d）,spleen（ 1,5 d）and kidney（ 1 d）（ P〈0. 05,P〈0. 01） while there were no obvious changes in plasma（ 12,21 d）,spleen（ 12,21 d）and kidney（ 5,12,21 d）（ P〉0. 05）. This study indicated that the gill and hepatopancreas were more vulnerable to Hg2 ＋than plasma,spleen and kidney,and their AKP and ACP actirities were not restored in the trial period.