CMV病毒对甜椒幼苗生理生化指标的影响

Effects of Cucumber mosaic virus(CMV) on physiological and biochemical indexes of sweet peppers

以两个抗性不同的甜椒品种为试材,苗期人工接种黄瓜花叶病毒病（CMV）,利用一步法RT-PCR技术对接种病毒类型进行检测,得到一条约780 bp的条带。而后对接种病毒幼苗在1～15 d内取样,测定植物电解质渗透率、丙二醛（MDA）含量、可溶性蛋白含量和抗氧化酶活性以及苯丙氨酸解氨酶（PAL）活性的变化,研究CMV病毒对甜椒幼苗生理生化指标的影响。结果表明：接种CMV病毒后,随着处理时间延长,两甜椒品种幼苗电解质渗透率和MDA含量都呈现总体升高的趋势,抗性较强的申椒1号比抗性弱的P14004增加幅度低。两品种的抗氧化酶活性除了超氧化物歧化酶（SOD）活性呈现下降趋势外,过氧化物酶（POD）和过氧化氢酶（CAT）活性均呈现总体上升趋势,且抗性较强的申椒1号增幅更大。而可溶性蛋白含量和PAL活性则都呈现先升高后降低的变化趋势,且两个指标都是申椒1号比P14004更高。

In order to elucidate the physiological and biochemical mechanism of sweet peppers infected by Cucumber mosaic virus（ CMV）,two different disease-resistant sweet peppers were used as materials. Firstly,we detected the inoculated virus types by one-step RT-PCR,and a 780 bp band was obtained. Then,we studied the changes of electrolytic leakage,malondialdehyde（ MDA） content,soluble protein content,the enzymatic activities of superoxide dismutase（ SOD）,peroxidase（ POD）,catalase（ CAT） and phenylalnine ammonialyase（ PAL） during 15 days. The results showed that： after CMV inoculation,while the treating time was prolonged,electrolytic leakage and MDA content exhibited an increasing trend as a whole,and the increase in Shenjiaoyihao with stronger resistance was lower than that in P14004 with weaker resistance. The antioxidant enzyme activities of both test plants increased,except that the SOD activity decreased,and the POD and CAT activity in Shenjiaoyihao showed higher increase rate than P12004. The soluble protein content elevated at first and reduced at last,so did the PAL activity in the two sweet pepper varieties,and both of the two indexes showed higher level in Shenjiaoyihao than in P14004.