摘要
病毒诱导的基因沉默(Virus induced gene silencing,VIGS)是一种转录后基因沉默现象,是植物体内普遍存在的遗传免疫机制,现已被开发为快速、高效、高通量的反向遗传学技术,在植物基因功能研究中得到广泛应用。近年,利用VIGS技术进行棉花基因功能研究也取得了一定进展。本文对VIGS技术的发展、操作技术的优化进行了综述,尤其总结了VIGS技术在棉花抗病、品质改良、生长发育等基因鉴定和功能研究中的应用进展,并对其应用前景进行了展望。
Virus-induced gene silencing（VIGS） is a kind of post-transcriptional gene silencing that is common in plants and has been widely applied to study gene functions as a reverse genetics technique because of its rapidity, high efficiency and throughput characteristics. In recent years, VIGS has been used in the research of gene functions in cotton. This paper reviews the principles of the VIGS technology system in cotton, especially summarizing the applications of VIGS in the identification and functional research of genes related to disease resistance, fiber quality, and development. Finally, the applications of VIGS in cotton research are discussed.
出处
《棉花学报》
CSCD
北大核心
2015年第5期469-473,共5页
Cotton Science
基金
国家科技重大专项"转基因生物新品种培育"(2014ZX0800501B-003)
棉花生物学国家重点实验室开放课题(CB2013A25)
