一种糖槭叶多糖的提取分离及结构分析

Isolation,Purification and Structure Characteristics Analysis of a Polysaccharide from Acer saccharum Marsh leaves

目的通过分离、纯化和结构特征解析,探讨糖槭叶多糖的结构特点。方法以糖槭叶为原料,采用热水提取的方法得多糖,阴阳离子串联树脂法脱蛋白,DEAE Cellulose 52柱色谱分离和Sephadex G-100色谱柱纯化,高效凝胶渗透色谱-蒸发光检测法测定多糖的纯度及分子量,高效液相色谱法、甲基化、气质联用色谱法、红外光谱、核磁共振光谱法和原子力显微镜研究糖槭均一多糖ASP-A-c结构。结果 ASP-A-c分子量为1.27×107Da,由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖和木糖以物质的量比5.39∶1.64∶1∶1.20∶1.5∶15.04∶2.87组成,共存α和β两种糖苷键,并以（1→4）连接的Galp构成主链,末端残基为Glcp和Galp,单链高度为2.0~9.4 nm。结论首次在糖槭中分离得到的ASP-A-c,并明确其结构。

Objective To isolate and purify the polysaccharide from Acer saccharum Marsh leaves and to analyse the structure characteristics. Methods The crude polysaccharide from hot water extraction was subject to remove protein and preliminary isolated through Amberlite FPA90- Cl-＋ Amberlite IRC- 84,purified by DEAE- cellulose 52 and Sephadex G- 100 chromatography,purity and molecular weight was determined by HPGPC- ELSD,a polysaccharide fraction named ASP- A- c was obtained. The structural characterization was identified by high performance liquid chromatography（ HPLC）,methylation,gas chromatography- mass spectrometer（ GC- MS）,Fourier transform infrared spectroscopy（ FTIR）,nuclear agnetic resonance spectroscopy（ NMR） and atomic force microscope（ AFM）. Results ASP- A- c was 1. 27 × 107 Da and it was mainly composed of mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose and xylose in the molar ratio of 4. 05. 39： 1. 64： 1： 1.20： 1. 5： 15. 04： 2. 87,glycosidic bond α and β; main chain was（ 1 →4）- linked Galp and the terminal residue was Glcp and Galp,single chain height is 2. 0 ~ 9. 4 nm. Conclusion ASP- A- c was first polysaccharide obtained from with Acer saccharum Marsh leaves and was understood the structure characteristics through experiment.