双孢蘑菇抗氧化肽的没食子酸化修饰及其体外抗氧化活性

Study on gallic acid-modified antioxidant peptide from Agaricus Bisporus and its antioxidant activities in vitro

采用没食子酸(GA)对双孢蘑菇抗氧化肽(ABAP)进行修饰,通过正交试验优化GA—ABAP的制备工艺,利用紫外—可见光谱及红外光谱证实了修饰反应的发生,同时通过体外试验模型比较ABAP修饰前后抗氧化活性变化。结果表明,GA—ABAP的最佳制备工艺为:GA与ABAP的体积比5∶1,pH 11.0,微波(起始700 W,沸腾后改为140 W)加热时间8min;该条件下制备的GA—ABAP的抗氧化活性较ABAP显著提高,还原力和对DPPH自由基的清除能力分别是修饰前的11.16和9.83倍,对Fe2+的螯合能力和对超氧阴离子自由基的清除能力分别比修饰前增加了78.00%和109.80%,说明GA修饰可有效提高ABAP的抗氧化活性。

With reducing power as the index of investigation,the preparation technology of gallic acid-modified antioxidant peptide fromAgaricus Bisporus（GA—ABAP）was optimized by of orthogonal test and in vitro antioxidant activities of GA—ABAP.The results showed that the optimum preparation conditions of GA—ABAP were as followed：volume proportion of GA to ABAP of 5∶1,pH value of11.0,heating time of microwave of 8min.The comparison showed that the in vitro antioxidant activities of GA—ABAP prepared in this condition were remarkably improved,the reducing power and DPPH radical scavenging activity of GA—ABAP were 11.16 and 9.83 times higher than those of ABAP,respectively;the metal chelating ability and superoxide anion radical scavenging ability of GA—ABAP were increased by 78.00% and 109.80%,respectively,and suggesting that GA modification may be effective in improving antioxidant activity of antioxidant peptides.