摘要
为了研究胰蛋白酶酶解瘤背石磺肌肉的最佳工艺,在单因素试验的基础上,通过响应面试验法和正交试验法比较最佳条件和蛋白提取率。结果表明:响应面试验法的最佳工艺条件为酶解温度44℃,酶解时间2.3h,pH 9.0,液固比92∶1(V∶m);而正交试验法的最佳工艺条件为酶解温度45℃,酶解时间2h,pH 9.0,液固比90∶1(V∶m),两种方法所得到的蛋白提取率分别为8.74%和8.51%。
The optimal enzymolysis processing for Onchidium struma muscle protein extraction by trypsin was investigated by response surface methodology and orthogonal design on the basis on the basis of single factor.The results showed that the optimum enzymolysis processing conditions via response surface methodology were as followed：enzymolysis temperature 45 ℃,enzymolysis time 2.3h,pH value 9.0and liquid-solid rate 92︰1（V ︰m）.And by the Orthogonal design,were enzymolysis temperature 45 ℃,enzymolysis time2 h,pH value 9.0and liquid-solid rate 90︰1（V ︰m）.The extraction protein rates by these of two methods were 8.74% and 8.52%respectively.
出处
《食品与机械》
CSCD
北大核心
2015年第5期226-230,共5页
Food and Machinery
基金
国家自然科学基金项目(编号:41276157)
关键词
瘤背石磺
蛋白
酶解
响应面法
正交试验法
Onchidium struma
protein
enzymolysis
response sur-face methodology
orthogonal design
