囊泡相关膜蛋白8在结肠癌中的表达及临床意义

Expression of vesicle-associated membrane protein 8 in colon cancer and its clinical significance

目的:探讨囊泡相关膜蛋白8(VAMP8)在结肠癌组织中的表达及其临床意义。方法:收集42例结肠癌患者癌组织及癌旁正常组织标本,用免疫组化法检测VAMP8蛋白的阳性表达率,分析其表达与结肠癌临床病理特征之间的关系;并选择其中12对标本,用q RT-PCR与Western blot法检测VAMP8 m RNA与蛋白的表达量。结果:q RT-PCR及Western blot检测显示,9对标本的结肠癌组织VAMP8 m RNA与蛋白表达量明显高于其对应的癌旁组织(均P<0.05);免疫组化结果显示,VAMP8在结肠癌组织中的阳性率明显高于癌旁组织(73.8%vs.42.9%;χ2=8.28,P<0.01),且VAMP8的表达与肿瘤的分化程度、Dukes分期及淋巴结转移情况有关(均P<0.05)。结论:VAMP8在结肠癌组织中表达上调,提示VAMP8可能通过调节自噬/溶酶体途径促进结肠癌的恶性进展与转移。

Objective：To investigate the expression of vesicle-associated membrane protein 8（VAMP8） in colon cancer tissue and its clinical significance.Methods：The specimens of colon cancer along with normal adjacent tissue from 42 patients were collected,in which the positive expression rate of VAMP8 protein was detected by immunohistochemical staining,and then the relations of VAMP8 expression with clinicopathologic characteristics of the patients were analyzed.Further,12 pairs of the specimens were selected,and then the VAMP8 mRNA and protein expression levels were determined by qRT-PCR and Western blot,respectively.Results：Results of qRT-PCR and Western blot analysis showed that the VAMP8 mRNA and protein expression levels in colon cancer tissue were significantly higher than those in their matched adjacent tissue in 9 pairs of specimens（both P〈0.05）.Results of immunohistochemical staining demonstrated that the positive expression rate of VAMP8 protein in colon cancer tissues was significantly higher than that in adjacent non-tumorous tissues（73.8%vs.42.9%;χ~2=8.28,P〈0.01）,and the VAMP8 expression was significant associated with histopathological grade,Dukes＇ stage and lymph node metastasis（all P〈0.05）.Conclusion：VAMP8 expression is increased in colon cancer tissue,which suggests that VAMP8 may promote the progression and metastasis of colon cancer through regulating the autophagy-lysosome pathway.