促血管生成素-2和血管内皮生长因子在寻常型银屑病血管生成机制中的相关性研究

Study on the Correlation between Angiopoietin-2 and VEGF in Patients with Psoriasis Vulgaris

目的:探讨促血管生成素-2(Ang-2)和血管内皮生长因子(VEGF)在寻常型银屑病皮损组织及外周血中的表达,研究二者在寻常型银屑病发病中的作用和意义。方法:随机选择30例寻常型银屑病患者,应用免疫组织化学法检测皮损中的Ang-2及VEGF蛋白的表达情况,双抗体夹心酶联免疫吸附法检测其外周血血清中Ang-2及VEGF的水平,并与30例正常人比较。结果:与对照组比较,寻常型银屑病患者皮损处的Ang-2和VEGF的表达明显增强(2值分别为9.64、20.12,P值均<0.01),血清中Ang-2及VEGF水平亦明显升高(t值分别为3.49、4.76,P值均<0.05)。二者血清浓度变化与PASI评分呈正相关(r值分别为0.59、0.77,P值均<0.01)。结论:Ang-2和VEGF在寻常型银屑病血管形成中扮演着重要作用,二者可能通过协同作用发挥相互促进作用。

Objective： To investigate the level of angiopoietin-2 and VEGF of psoriatic lesions and serum in patients with psoriasis vulgaris,and to explore the significance in the pathogenesis of psoriasis. Methods： The expression of angiopoietin-2 and VEGF in skin lesions of 30 patients with psoriasis vulgaris and 30 normal controls were detected by immunohistochemistry technique. Levels of angiopoietin-2 and VEGF in sera were measured by double antibody sandwich enzyme-linked immunosorbent assay（ ELISA） in patients with psoriasis vulgaris and normal controls. Results：The expression of angiopoietin-2 and VEGF in psoriatic lesions were remarkable higher than those in normal controls（ 2= 9. 64,20. 12,both P 〈 0. 01）. Serum level of angiopoietin-2 and VEGF were significantly higher in patients with psoriasis than those in normal controls（ t = 3. 49,4. 76,both P 〈 0. 05）. The level of angiopoietin-2 and VEGF in serum were associated with PASI scores（ r = 0. 59,0. 77,both P 〈 0. 01）. Conclusion： Angiopoietin-2 and VEGF play an important role in the angiogenesis in psoriasis. Both may play a role in promoting mutual by synergistic effect.