西拉葡萄浆果代谢物对低海拔和高海拔气象因子的响应

Shiraz/Syrah Grape Metabolites Response to Low/High Altitudes

以酿酒葡萄西拉为材料,采用GC-TOF-MS技术解析低海拔(41 m)和高海拔(2 278 m)产区气象因子对西拉浆果代谢组的影响。结果表明:2 278 m产区的品质高于41 m产区,代谢组分析表明2 278 m产区西拉浆果上调22种代谢物,下调11种代谢物;代谢通路表明:2 278 m产区西拉积累更多的风味物质、增强营养价值和人类保健物质;通路富集发现西拉葡萄浆果通过调整氨基酸、碳水化合物、脂质、氮代谢通路来适应2 278 m产区下的逆境;DCCA分析表明环境因子中的日均紫外辐射、生长时期总辐射和日均辐射是调控西拉果实代谢物的主要因子,因此西拉通过代谢物和代谢通路的多样性策略来适应高海拔环境,提高果实品质。

Shiraz/Syrah grape as experiment material in this study, GC-TOF- MS based technique employed for analytical low altitude （41 m）and high altitude （2 278 m ） metabolism. The results showed that Shiraz/Syrab qualities in 2 278 m region were higher than that in 41 m region, the metabolism analysis showed that 2 278 m region up-regulated 22 kinds of metabolites of Syrab berries ,down-regulated 11 kinds of metabolites. The metabolic pathways：2 278 m region accumulation of flavor substances,enhanced nutritional value and human health care materials;pathway enrichment under the 2 278 m region grape berries by adjusted amino acids ,carbohydrate ,lipids ,nitrogen metabolism pathway to adapt to the 2 278 m region abiotic stress;DCCA analysis showed that the environmental factors such as daily UV radiation, growth period total radiation and daily radiation were main factors controlling Shiraz/Syrab fruit metabolites acccumulation, therefore Shiraz/Syrah through metabolites and metabolic pathways diversity strategies to adapt to high altitude physical environment, enhenced the fruit quality.