摘要
In the present study, we aimed to determine the major components responsible for the analgesic effects of Dahurian Angelica root. The rats were administered with either the volatile oil or decoction of Dahurian Angelica root. Formalin plantar injection test was employed as the pain model. The rats were observed for nociception, after which their blood was collected from the abdominal aorta. High-performance liquid chromatography （HPLC） fingerprints of serum were performed. The results demonstrated that no remarkable analgesic effect was observed from the low-dose decoction group, whereas the other doses showed pain-relieving effects in the rat model. The components of the rat sera in the different treatments were determined by HPLC. Common chromatographic peaks were observed at 24.941, 25.755, 29.854, 32.761 and 33.928 min, representing the major constituents of the decoction and volatile oil of Dahurian Angelica root. The analgesic components of Dahurian Angelica root obtained from the rat serum mainly peaked at 24.941, 25.755, 29.854, 32.761 and 33.928 min, suggesting trace components and metabolites.
本文旨在分析白芷镇痛的主要化学成分。大鼠足底注射甲醛致痛造模,腹腔灌注白芷精油和水煎液高、中、低剂量组,观察各组大鼠疼痛反应,然后腹主动脉取血,高效液相色谱(HPLC)分析大鼠血清。结果显示:白芷水煎液低剂量组镇痛不明显外,其余各组对模型大鼠都有镇痛作用。24.941、25.755、29.854、32.761和33.928 min的色谱峰是各组模型大鼠血清的公共峰,它们来自白芷精油和水煎液[5]。白芷镇痛成分可能是色谱峰24.941、25.755、29.854、32.761和33.928 min所示化合物,及其一些微量成分和代谢产物。
