高功率微波对大鼠脑组织氧化应激的影响

Oxidative stress effect of HPM on brain of rats

目的观察不同剂量高功率微波(high power microwave,HPM)辐照对Wistar大鼠脑组织的损伤作用及氧化应激的影响,为建立微波辐射氧化损伤动物模型及抗辐射药物的评价提供依据。方法将80只健康成年雄性Wistar大鼠按析因设计随机分为16组,辐射平均功率密度分别为0、10、30及100 m W/cm2;取材时间分别为6 h、1、3及7 d。照射组照射时间为6 min。分别于各取材时间点活杀动物并取脑组织,采用比色法测量大鼠脑组织丙二醛(MDA)含量、蛋白质羰基含量和谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性。结果各辐射组MDA、蛋白质羰基含量基本随着辐射剂量的升高而升高,随取材时间的延长而降低。各辐射组SOD、GSH-px、CAT活性基本随辐射剂量的升高而降低,随取材时间的延长先降后升。结论微波辐射能引起大鼠脑组织的氧化应激反应,主要表现在大鼠脑组织脂质和蛋白质氧化损伤以及抗氧化物酶活性降低,且与辐射剂量、取材时间有一定的依赖性。

Objective To investigate the adverse effect of different doses of high power microwave（HPM） irradiation on oxidative stress in the brain of Wistar rats in order to contribute to establishing an animal model to evaluate protective agents which will be used for protection against microwave radiation.Methods Eighty male Wistar rats were randomly divided into 16 groups according to factor analysis.The average power density was 0,10,30 and 100 mW/cm2 and the sampling time was 6 h,1,3 and 7 d .The duration of exposure was 6 minutes for each radiation group.After exposure, the rats were sacrificed at each sampling time.Colorimetric method was used to measure the content of malondialdehyde（MDA） and protein carbonyl, the activity of GSH-px, SOD and CAT.Results The content of MDA and protein carbonyl of each radiation group was increased with the radiation dose, but decreased with the sampling time prolonged.The activity of superoxide dismutast（SOD）,glutathion peroxidase（GSH-px） and catalase（CAT） in each radiation group was decreased with the radiation dose increased, and with the sampling time prolonged, but increased later.Conclusion Microwave radiation can cause oxidative stress in rats brain, as shown by the oxidative damage of lipid and protein and the decrease in the activity of antioxidant enzymes.Besides, the effect also depends on the radiation dose and sampling time.