HPLC测定冠心丹参胶囊中主成分的含量

Determination of Main Components in Guanxin Danshen Capsules by HPLC

目的：测定冠心丹参胶囊中丹参素、丹酚酸B、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA以及人参皂苷Rg1和人参皂苷Rb1的含量。方法：采用高效液相色谱法，以C18柱为色谱柱；流动相为乙腈-0.1％磷酸，梯度洗脱；流速为0.8 mL·min-1；检测波长为270、203 nm。结果：丹参素钠和丹酚酸B、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA以及人参皂苷Rg1和人参皂苷Rb1分别在7.1~113.6、11.7~187.2、6.6~105.0、6.9~110.2、7.3~116.0、31.2~499.4、27.1~433.7 μg·mL-1呈良好线性关系，平均加样回收率分别是99.51%（RSD=0.91%）、97.73%（RSD=1.35%）、97.20%（RSD=0.78%）、98.47%（RSD=0.69%）、99.12%（RSD=0.31%）、96.64%（RSD=0.86 %）和97.98%（RSD=1.02%）。结论：本方法简便、准确、重复性好，可作为冠心丹参胶囊中主成分的含量测定方法。

Objective：To determine the content of the tanshinol natrium，salvianolic acid B，cryptotanshinone，tanshinoneⅠ，tanshinoneⅡA，ginsenoside Rg1 and ginsenoside Rb1 in Guanxin Danshen Capsules.Methods：HPLC was performed on C18 column using acetonitrile-0.1% phosphoric acid （gradient elution） as the mobile phase at a flow rate of 0.8 mL·min-1.The detection wavelengths were set at 270 nm and 203 nm.The column temperature was 30 ℃.Results：Tanshinol natrium，salvianolic acid B，cryptotanshinone，tanshinoneⅠ，tanshinoneⅡA，ginsenoside Rg1 and ginsenoside Rb1 showed good linearity in the concentration range of 7.1-113.6，11.7-187.2，6.6-105.0，6.9-110.2，7.3-116.0，31.2-499.4 and 27.1-433.7 μg·mL-1，respectively.And their average recovery rates were 99.51% （RSD=0.91%），97.73% （RSD=1.35%），97.20% （RSD=0.78%），98.47% （RSD=0.69%），99.12% （RSD=0.31%），96.64% （RSD=0.86%） and 97.98% （RSD=1.02%），respectively.Conclusion：The method is simple and accurate with good reproducibility.It can be used for the determination of the main components in Guanxin Danshen Capsules.