激活处理对人卵子和早期胚胎ATP含量影响的初步研究

Investigation of the ATP Content in Human Unfertilized Oocytes and Early-stage Embryos after Activation

目的:探讨激活处理对人未受精卵子和早期胚胎三磷酸腺苷(ATP)含量影响。方法:收集辅助生殖治疗周期中体外受精(IVF)以及单精子卵母细胞浆内注射(ICSI)时的未受精卵子(包括GV期、MⅠ期、MⅡ期卵母细胞),以及早期胚胎(2-cell、4-cell胚胎),采用生物化学发光法测定在未处理和进行激活处理后的ATP含量。结果:1未处理的GV期、MⅠ期、MⅡ期卵子和2-cell、4-cell期胚胎的ATP含量分别为1.14±0.15、1.41±0.14、1.45±0.12、1.61±0.25、2.05±0.23 pmol,激活处理后GV期、MⅠ期、MⅡ期卵子和2-cell、4-cell期胚胎的ATP含量分别为1.60±0.17、1.86±0.25、2.06±0.38、2.26±0.53、2.55±0.46 pmol;2激活处理后GV期、MⅠ期卵子的ATP含量显著性高于未处理组(P<0.05)。结论:人GV期、MⅠ期、MⅡ期卵子和2-cell、4-cell期胚胎中ATP含量逐渐增加;经激活处理后,仅GV期、MⅠ期卵子的ATP含量有显著性变化。

Objective： To investigate the impact of activation to the ATP content in human unfertilized oocytes and early-stage embryos. Methods： The unfertilized oocytes（include germinal vesicle, metaphase I, metaphase II oocytes） and early-stage embryos（include 2-cell, 4-cell embryos） from IVF and ICSI in assisted reproduction cycles, were detected by ATP-bioluminescence assay to analyzed the ATP content before and after activation. Results： 1 The ATP content of human GV, MI, MII oocytes and 2-cell,4-cell embryos in untreated and activated group were 1.14 ±0.15, 1.41 ±0.14, 1.45 ±0.12,1.61 ±0.25, 2.05 ±0.23 pmol, and 1.60 ±0.17,1.86±0.25, 2.06±0.38, 2.26±0.53, 2.55±0.46 pmol. 2The ATP content of GV and MI oocytes in activated group was significantly higher than that in untreated group（P〈0.05）. Conclusion： The ATP content of human GV, MI, MII oocytes and 2-cell, 4-cell embryos is keep increasing; Only the ATP content of GV and MI oocytes after activation treatment were significantly higher than the untreated group.