间歇低氧对大鼠下丘脑-垂体-性腺轴功能和超微结构的影响及还原型谷胱甘肽的干预作用

The effects of intermittent hypoxia on the function and ultrastructure of hypothalamus-pituitary- gonadal axis in rats and the protective influence of reduced glutathione

目的研究间歇低氧对大鼠下丘脑-垂体-性腺轴（HPG）功能和超微结构的影响及还原型谷胱甘肽（GSH）的保护作用，探讨睡眠呼吸暂停低通气综合征所致男性性功能障碍的发病机制。方法将24只成年雄性sD大鼠随机分为3组：常氧对照组（Nc）、间歇低氧组（IH）、间歇低氧＋还原型谷胱甘肽干预组（IH＋GSH），每组8只。4周后用放射免疫法检测各组大鼠血清促卵泡生成素（FSH）、促黄体生成素（LH）、睾酮（T）、白细胞介素（IL）-6、肿瘤坏死因子（TNF）-α；比色法检测血清丙二醛（MDA）含量、超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GSH-Px）活性。电镜观察各组大鼠下丘脑、垂体、睾丸的超微结构。结果与Nc组大鼠比较，IH组血清FSH、LH、T水平明显降低，MDA、IL-6、TNF-α含量升高，SOD、GSH-Px活性降低，差异有统计学意义（P〈0．05）；IH组大鼠下丘脑、垂体、睾丸组织超微结构损伤重；IH＋GSH组与IH组比较各指标有改善；IH＋GSH组超微结构损伤较轻。结论间歇低氧可通过氧化应激引起大鼠HPG轴功能及细胞超微结构损伤，GSH具有一定的保护作用。

Objective To study the effects of intermittent hypoxia（IH） on the function and ul- trastructure of hypothalamus-pituitary-gonadal（HPG） axis in rats and the protective influence of reduced glutathione, and to explore the pathogenesis of male sexual dysfunction caused by sleep apnea hypopnea syndrom. Methods 24 healthy adult Sprague-Dawley male rats were randomly divided into three groups （ n = 8 ）, normoxia control group （ NC ）, intermittent hypoxia group （IH） and intermittent hypoxia combined glutathione intervention group （ IH ＋ GSH）. After 4 weeks, the level of follicle stimulating hormone （ FSH）, luteinizing hormone （ LH ）, testosterone （ T）, interleukin （IL） -6, tumor necrosis factor （TNF） -or in serum were measured by radioimmunity method in all rats. Colorimetric method were used to detect concentration of malondialdehyde（ MDA）, superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in serum. Furthermore, the ultrastructures of hypothalamus, pituitary and testis were observed by transmission elec- tron microscope. Results Compared with NC,the level of serum FSH,LH and T were significantly lower in IH group, while the concentrations of MDA, IL-6 and TNF-ct were significantly higher, while SOD and GSH-Px activity decreased significantly. Compared with IH group, the level of serum biochemical indices improved in IH ＋ GSH. Compared with NC, the ultrastructure of hypothalamus, pituitary and testis tissues damaged seriously in IH group,whereas these damaged to a lesser degree in IH ＋ GSH. Conclusion IH can cause the disruption of the function and ultrastructure of HPG axis by oxidative stress in rats. GSH has certain protective effect to it.