堆肥放线菌DF02分离及对番茄灰霉病生防作用

Isolation of Actinomycete DF02 from Composting and Its Application in Biological Control of Botrytis cinerea

目的:从堆肥样品中分离具有生防作用的放线菌,并对其进行菌种鉴定及对番茄灰霉病菌(Botrytis cinerea)的盆栽防治效果进行研究。方法:采用稀释涂布法分离堆肥样品中的放线菌,采用混菌法和牛津杯法研究堆肥样品中放线菌的抑菌活性,根据形态观察、生理生化特性和16S r DNA分析对抗菌活性较强的菌株进行菌种鉴定,并研究该菌株的盆栽防治效果。结果:从堆肥样品中共分离到放线菌31株,有3株放线菌对指示菌株具有抑制作用;其中放线菌DF02菌株对供试菌中的5种植物病原真菌均有拮抗作用,且对番茄灰霉病菌的抑制作用较强;盆栽试验结果显示,DF02菌株发酵液上清对番茄灰霉病的保护效果和治疗效果分别是58.47%和53.83%;分类地位研究表明,DF02菌株与Streptomyces neopeptinius最接近,初步将其鉴定为Streptomyces neopeptinius。结论:DF02是一株具有研究开发潜力的生防放线菌菌株,本研究结果为番茄灰霉病的生物防治提供了实验依据。

Objective：To isolate and identify the biocontrol actinomycete from composting, and to investigate its potted control effect against Botrytis cinerea. Methods：Actinomycetes were isolated from composting by pour plate method. Inhibitory activities of actinomycetes were determined by dilution plate method and cylinder plate method. Strain with high activities was identified based on morphology and biochemical characterization and 16S rDNA gene sequence analysis. And its potted control effect was also investigated. Results：31 strains of actinomycetes were isolated, three of them had inhibitory effects on the test strains. Of which, the strain DF02 showed antagonistic to five plant pathogenic fungi of all the tested strains, and its inhibitory effect on Botrytis cinerea was the strongest. The resuhs of potted control effect test showed that the protective and therapeutic efficacy of the fermentation suspension to Botrytis cinerea was 58.47% and 53.83% , respectively. According to taxonomic identification, the strain DF02 was identified as Strepto- myces neopeptinius. Conclusion：Straln DF02 is a biocontrol actinomycete with broad development potential, and these results provide ex- perimental basis for the biocontrol of Botrytis cinerea.