摘要
目的:研究麻花秦艽、粗壮秦艽、粗茎秦艽、西藏秦艽等藏药解吉4种高山基原植物的种胚发育特性。方法:采用培养皿培养法,观察记录种子发芽率、测量种胚各部位长度和宽度,进行统计分析;采用石蜡切片法,观察淀粉粒、叶绿体生成情况。结果:4种植物的发芽率均很高;种胚发育特性基本一致,主要在时间长度上存在差异;胚长度的增长主要体现为胚轴的增长;细胞中贮藏淀粉粒;子叶中逐渐产生叶绿体;并将其种胚发育生长过程分为8个时期。结论:本研究可为相关种群的就地保护、遗传育种、药材栽培学提供基础资料。
Objective：To study the embryonic development of C, entiana straminea, G. robusta, G. crassicaulis and G. tibetica. Methods ： The seed germination rates,length and width of embryos, starch grains and chloroplasts were observed and analyzed by statistic soft- ware. Results ： The seed germination rates of the four .species were all high. The increase of the length of embryo depended mainly on the increase of the length of hypocotyl. Starch grains were stored in cells and chloroplasts appeared in cotyledons. The process of germination was divided into eight periods. Conclusion：The embryo growth characteristics of the four species are recognized, and the results can be used to study the in situ conservation, genetic breeding and cultivation of Sect. Cruciata.
出处
《中药材》
CAS
CSCD
北大核心
2015年第8期1571-1575,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81173654)
关键词
龙胆属
解吉
种胚发育
叶绿体
淀粉粒
C, entiana genus
Jie-ji
Embryonic development
Chloroplast
Starch grain
