竹节参总皂苷通过线粒体途径保护H_2O_2诱导的SH-SY5Y神经细胞损伤

Protective Effects of Total Saponins from Panax japonicus on H_2O_2 Induced Injury via Mitochondia Pathway in SH-SY5Y Cells

目的:探讨竹节参总皂苷对SH-SY5Y细胞的保护作用机制。方法:SH-SY5Y细胞随机分为正常对照组,H2O2模型组(600μmol/L H2O2),药物干预组(600μmol/L H2O2+0.1、1、5、20μg/m L竹节参总皂苷)。竹节参总皂苷预处理12 h后,再加入H2O2继续培养12 h。JC-1法检测线粒体膜电位变化,Western blotting检测Sirt1、PGC-1α、Foxo3a、LC3-Ⅱ和Beclin1的表达。结果:600μmol/L H2O2与SH-SY5Y共同孵育12 h后,线粒体膜电位下降,Sirt1、PGC-1α、Foxo3a、LC3-Ⅱ和Beclin1的蛋白表达下调,预孵育竹节参总皂苷能明显提高细胞线粒体的膜电位,增强Sirt1、PGC-1α、Foxo3a、LC3-Ⅱ和Beclin1的蛋白表达。结论:竹节参总皂苷对H2O2致SH-SY5Y神经细胞损伤具有保护作用,其机制可能与调节线粒体功能及自噬相关蛋白有关。

Objective：To investigate the protective mechanism of total saponins from Panax japonicus （SPJ） on H202 induced injury in SH-SYSY cells. Methods：SH-SYSY cells were divided into three groups：blank control group, model group（600μmol/L H2O2 ）and drug treatment groups. Different concentrations of SPJ（0. 1,1,5 and 20 μg/mL）were incubated with SH-SY5Y cells for 12 hours prior to exposing to 600 μmol/L H202 for another 12 h. Mitochondrial membrane potential（MMP） was detected by JC-1 method. Protein expressions of Sirtl, PGC-1α, Foxo3a, LC3-Ⅱ and Beclinl were detected by Western blotting. Results： Compared to the H2O2 model group, SPJ pretreatment significantly increased MMP level and enhanced the protein expressions of Sirtl, PGC-1α, Foxo3a, LC3-Ⅱ and Beclinl. Conclusion ：SPJ exerts orotective effect on H, O, induced SH-SY5Y cell iniurv through mitochondria nathwav.