ESI-MS研究反药配伍对乌头汤肠内菌代谢的影响

Study on Effects of Incompatible Herbal Medicine on Intestinal Bacterial Metabolism of Wu-Tou-Tang by ESI-MS

目的:利用ESI-MS技术对乌头汤及其分别与半夏、浙贝母、川贝母配伍的煎煮液经肠内菌代谢前后的样品进行检测。方法:根据各药液中生物碱成分经肠内菌代谢前后相对丰度比的变化,分析反药配伍对乌头汤中生物碱成分肠内菌代谢的影响。结果:半夏、浙贝母、川贝母均抑制了乌头汤中双酯型生物碱的肠内菌生物转化,说明以上3种中药与乌头汤配伍均有增毒作用。结论:从肠内菌代谢的角度揭示中药"十八反"的科学内涵。

Objective：Electrospray ionization-mass spectrometry（ESI-MS） was used to detect samples of Wu-Tou-Tang as well as the co-decoction of it with Pinelliae Rhizoma, Fritillariae Thunbergii Bulbus and Fritillariae Cirrhosae Bulbns before and after intestinal bacterial metabolism. Methods ： Semi-quantitative method of mass spectrometry was established for analyzing the biotransformation of ma- jor chemical constituents in Chinese medicine formula. Based on the changes of relative abundance ratio of alkaloids to internal standard for each decoction before and after intestinal bacterial metabolism, effects of incompatible herbal medicine on the intestinal bacterial me- tabolism of aconite alkaloids from Wu-Tou-Tang were analyzed. Results：Pinelliae Rhizoma, Fritillariae Thunbergii and Fritillariae Cir- rhosae Bulbus inhibited the intestinal bacterial biotransformation of diester-alkaloids, which could be considered that the above three Chinese herbal medicines increased the toxicity of Wu-Tou-Tang. Conclusion ：This study illustrates the scientific connotation of tradition- al Chinese medicine ＂eighteen incompatible medicaments＂ theory from the point of intestinal bacterial metabolism.