包涵体蛋白复性的研究进展

The Research Progress of Inclusion Body Protein Renaturation

包涵体是指外源重组蛋白在细胞内凝集的无活性的固体颗粒。经分离、洗涤除去一些膜蛋白或膜碎片后,用适宜试剂溶解,如高p H值加低浓度的尿素缓冲液、不同羟基的醇类或含小分子的变性剂。传统的稀释、透析等复性方法效率一般较低,目前可通过高静水压、沸石的高通量筛选及高通量筛选结合实验设计软件的方法提高复性效率。希望可以找到一种适于所有重组蛋白的高效、简便的复性方法,实现不易获得的外源重组蛋白简易的规模化生产。

Inclusion bodies are the inactive solid particles agglutinating by exogenous recombinant pro- tein in cells. After separation and washing to remove some pieces of membrane proteins or film, they can be dissolved by appropriate reagent, for example the buffer with high pH value and low concentration of urea, alcohol with different hydroxyl or denaturant containing small molecule. The renaturation efficiency traditional renaturation method such as dilution, dialysis is generally low. At present, we can improve refolding efficiency through high pressure, high throughput screening of zeolite and high-throughput screening combined with experimental design software. We wish to find an efficient renaturation method suitable for all recombinant proteins, so as to realize large-scale production of recombinant proteins which are difficult to obtain.