不同斑蝥素类物质对人肝癌细胞HepG_2增殖作用的研究

Effect of different cantharidin materials on proliferation of human hepatocellular carcinoma cells HepG2

目的观察斑蝥素酸镁、斑蝥素及斑蝥素酸钠3种斑蝥素类物质对人肝癌细胞Hep G2的体外抗肿瘤活性。方法 (1)采用磺酰罗丹明染色法(SRB法)检测不同浓度斑蝥素酸镁、斑蝥素及斑蝥素酸钠在体外对人肝癌细胞Hep G2增殖的抑制作用;(2)使用透射电镜观察2.27μmol/L斑蝥素酸镁作用于肝癌细胞Hep G248 h后细胞超显微结构的变化。结果 (1)斑蝥素酸镁、斑蝥素及斑蝥素酸钠对人肝癌细胞Hep G2有明显抑制作用,抑制率随药物浓度的增加而升高,且呈剂量效应关系,其半数抑制浓度(IC50)分别为2.27、5.70、8.41μmol/L;(2)透射电镜下见核染色质聚集成块附着于核膜下,且见核异形等凋亡细胞形态特征。结论斑蝥素酸镁对人肝癌细胞Hep G2的抑制作用优于斑蝥素和斑蝥素酸钠,且斑蝥素酸镁可以诱导人肝癌细胞Hep G2发生凋亡。

Objective To investigate the in vitro anti-tumor activity of three kinds of cantharidin materials magnesium cantharidate,cantharidin and sodium cantharidate on hepatocellular carcinoma cells Hep G2. Methods（1）The sulforhodamine B（SRB） assay was employed to evaluate the inhibitory effect of magnesium cantharidate,cantharidin and sodium cantharidate on hepatocellular carcinoma cells Hep G2 in vitro;（2）the transmission electron microscopy was used to observe the ultra-structural changes after 2.27 μmol/L magnesium cantharidate acting on hepatocellular carcinoma cells Hep G2 for 48 h. Results（1）Magnesium cantharidate,cantharidin and sodium cantharidate had obvious inhibitory effects on the proliferation of hepatocellular carcinoma cells Hep G2,the inhibitory rate was increased with the medication concentration increase,showing the dose-effect relationship,the half inhibitory concentrations（IC50） were 2.27,5.70,8.41 μmol/L,respectively;（2）the transmission electron microscopy found the morphological features of apoptotic cells,such as the nuclear chromatin was gathered into a block attaching to below the nuclear membrane,heteromorphic nucleus and so on. Conclusion The inhibiting effect of magnesium cantharidate on hepatocellular carcinoma cells Hep G2 is superior to that of cantharidin and sodium cantharidate,moreover magnesium cantharidate can induce the apoptosis of hepatocellular carcinoma cells Hep G2.