正交实验优化紫叶甘蓝型油菜花色苷提取工艺

Optimization Technology of Extracting Anthocyanins From the Purple Leaf Brassica napus by Orthogonal Experiment

以紫叶甘蓝型油菜为试材,采用正交实验设计方法,研究提取温度、提取剂种类、提取时间、料液比等对紫叶甘蓝型油菜花色苷提取率的影响,同时建立最佳提取工艺。结果表明:影响紫叶甘蓝型油菜花色苷提取率的因素依次为提取时间>料液比>提取温度>提取剂种类,其最佳提取工艺为提取温度60℃,提取剂3%盐酸+90%乙醇,料液比1∶15g/mL、提取时间5h。

Taking purple leaf Brassica napus as the research materials,the ortho-design experiment was carried out to study the influence of extraction time,extraction temperature,extraction agent and solid-liquid ratio on purple leaf Brassica napus anthocyanins extraction yield,and to establish the best extraction process.The results showed that factors which affected the anthocyanins extraction yield of purple leaf Brassica napus were extraction timesolid-liquid ratioextracting temperatureextracting agent type,and the optimum technology parameters were follows,the extracting temperature was 60℃;extracting agent was 3% hydrochloric acid＋90%ethanol;solid-liquid ratio was 1∶15g/mL and the extracting time was 5h.