酶法检测血清游离脂肪酸的方法学评价

Methodological evaluation of enzymolysis to detect serum free fatty acids

目的评价酶法检测血清游离脂肪酸的方法学。方法参照美国临床和实验室标准化协会（CLSI）推荐的评价方法,对酶法测定血清游离脂肪酸的精密度、比对试验、回收试验、干扰试验、线性范围进行评价。结果精密度：酶法测定血清游离脂肪酸的精密度批内、批间、日间、总变异系数（CV）均〈5%,精密度好;比对试验：2种厂家的试剂盒测定血清游离脂肪酸（FFA）结果相关性良好（r2=0.9821,n=40）,测定结果基本一致（t=0.08,P〉0.05）;干扰试验：在血红蛋白2g/L、胆红素342μmol/L、甘油三酯37mmol/L、维生素C170μmol/L的范围内,酶转法测定血清游离脂肪酸干扰相对偏差〈10.0%;平均回收率为101.25%;线性范围为0.3-3.3mmol/L,线性良好。结论酶法测定血清游离脂肪酸的精密度、正确度、抗干扰能力及线性范围可满足临床需求,适合在临床实验室使用。

Objective To evaluate methodology for enzymolysis to detect serum free fatty acids（FFA）.Methods The accuracy,comparative test,interference test,recovery test and linear range of serum FFA with enzymic method were evaluated in accordance with the clinical and laboratory standards institute and established method in our laboratory.Results Precision was high to measure serum FFA using enzymic method,a right correlation was observed to determine Hey between enzymic method and circulating enzymatic determination（r2=0.9821,n=40）,and the results remained accordance（t=0.08,P 〉0.05）.The results of interference test showed that relative deviation,recovery rate and linear range were10.0%,101.25%,and 0.3-3.3mmol/L respectively using enzymic method to measure serum FFA when hemoglobin（2g/L）,bilirubin（342μmol/L）,triglycerides（37 mmol/L）and vitamin C（170μmol/L）were considered to be several interference factors.Conclusion Our results suggest that enzymatic determination to test serum FFA is able to meet clinical requirement,as well as it is suitable in the clinical laboratories for precision,accuracy,anti-interference performance and linear range.