草乌花总生物碱的纯化工艺研究

Study on Purification Technology of Total Alkaloid from the Flos of Aconitum kusnezoffii

目的:优选草乌花总生物碱的纯化工艺。方法:采用酸碱滴定法测定草乌花总生物碱的含量。以树脂型号、上样药质量浓度、交换速度为考察因素,以最大吸附量、解吸率和总生物碱质量分数为指标,优化离子树脂纯化草乌花总生物碱的工艺并进行验证。结果:选择树脂型号为732型阳离子交换树脂、上样液质量浓度为0.32 g/L、交换速度为7倍柱体积(BV)/h为最优纯化工艺。验证试验中纯化后总生物碱质量分数平均值为86.88%(RSD=0.52%,n=3),解吸率平均值为92.81%(RSD=0.40%,n=3);3批样品草乌花总碱提取转移率平均值为81.76%,纯化转移率平均值为89.47%。结论:建立的纯化工艺稳定、可行,且转移效率较高。

OBJECTIVE：To optimize the purification technology of total alkaloid from the flos of Aconitum kusnezoffii. METHODS：The content of total alkaloid from the flos of A. kusnezoffii was determined by acid-base titration. The purification technology of total alkaloid from the flos of A. kusnezoffii was optimized by ion resin with resin type,mass concentration of loading liquid and exchange speed as factors,maximum adsorption quantity,desorption rate and mass fraction of total alkaloid as index,and verification test was conducted. RESULTS：The optimal purification technology was as follows as type 732 cation exchange resin,mass concentration of loading liquid 0.32 g/L,exchange speed of 7 column volume（BV）/h. In validation test,the content of total alkaloid was 86.88%（RSD=0.52%,n=3）,and desorption rate was 92.81%（RSD=0.40%,n=3）averagely. The extraction transport rate of total alkaloid from 3 batches of the flos of A. kusnezoffii was 81.76% and purification transport rate was 89.47% in average. CONCLUSIONS：The established method is stable and feasible,and shows high transport rate.