寡聚化和磷酸化α-突触核蛋白在不同年龄段食蟹猴脑不同部位突触小体中的表达分布

Differential expressions of oligomer α-synuclein and phosphorylated α-synuclein in synaptosome in the brain regions of different ages of cynomolgus monkeys of different ages

目的研究寡聚化(oligomer-α-synuclein,oligo-α-Syn)和磷酸化α-突触核蛋白(phosphorylated-α-Synuclein,p-α-Syn)在不同年龄段食蟹猴脑不同部位突触小体中的表达差异。方法酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测oligo-α-Syn与p-α-Syn在不同年龄段食蟹猴脑不同部位突触小体中的表达。结果在老年食蟹猴的前额叶、枕叶、颞叶、海马、纹状体和丘脑中,突触小体中寡聚化与磷酸化α-Syn表达较青年与中年猴显著增加,且差异有统计学意义(P<0.01)。结论食蟹猴颞叶、海马、纹状体和丘脑突触小体中α-Syn寡聚化与磷酸化表达呈增龄性增加。

Objective To explore the expressions of oligomer α-synuclein（ oligo-α-syn） and phosphorylated α-synuclein（ p-α-syn）in synaptosome in brain regions of cynomolgus monkeys of different ages. Methods ELISA was applied to measure the expressions of oligo-α-syn and p-α-syn in different brain regions of cynomolgus monkeys of different ages. Results The synaptosome-α-Syn oligomers and synaptosome-p-α-syn increased significantly in prefrontal lobe,occipital lobe,temporal lobe,hippocampus,striatum and thalamus of old monkeys compared with that of young and middle-aged monkeys（ P〈0. 01）. Conclusion The highest level expression of oligo-α-syn and p-α-syn was found in synaptosome of all brain regions in old cynomolgus monkeys.