胡桃醌脂质体的制备及理化性质

Development and characterization of Juglone liposomes

采用乙醚注入法制备胡桃醌脂质体,在单因素试验基础上,以膜材比、脂药比、注入速度为影响因素,包封率为响应值,利用Box-Behnken试验设计原理,采用3因素3水平的响应面分析法,对胡桃醌脂质体制备工艺进行优化。胡桃醌脂质体的最佳制备条件为膜材质量比4.86∶1.00,脂药质量比10.92∶1.00,注入速度1.52 m L·min-1,采用最佳条件制备的胡桃醌脂质体包封率较高,验证实验测得胡桃醌脂质体的包封率为79.80%,与模型预测值相对误差为0.033%,重现性好。

Juglone liposomes were prepared by ether injection method. One-factor-at-a-time design was used to investigate the effects of five process parameters on encapsulation efficiency. Three main process parameters that influence encapsulation efficiency including lecithin / cholesterol mass ratio,lecithin / juglone mass ratio and injection speed were optimized by response surface analysis based on a three-variable and three-level Box-Behnken experimental design. The optimal conditions for preparing juglone liposomes were determined as 4. 86∶1. 00 of lecithin /cholesterol mass ratio,10. 92∶1. 00 of lecithin / juglone mass ratio and 1. 52 mL·min^- 1of injection speed. Under these conditions,the actual encapsulation efficiency of juglone liposomes was 79. 80%,which showed a relative error of 0. 033% when compared with the predictive value.