HPLC法测定血塞通软胶囊中三七皂苷R_1及人参皂苷Rg_1、Re、Rb_1的含量

Content determination of notoginsenoside R_1, ginsenoside Rg_1, Re, Rb_1 in Xuesaitong soft capsules by HPLC

目的：建立高效液相色谱法测定血塞通软胶囊中三七皂苷R1、人参皂苷Rg1、Re、Rb1含量的方法,为制定该制剂质量标准提供参考。方法：采用HPLC法,选用ZORBAX SB-Aq色谱柱（250 mm×4.6 mm,5μm）;流动相为乙腈-水梯度洗脱;流速：1.0 m L·min-1;检测波长：203 nm;柱温：25℃。结果：三七皂苷R1、人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1分别在0.32-3.20μg、0.72-7.20μg、0.21-2.10μg、0.92-9.20μg范围内呈良好线性关系;相关系数分别为1.000 0、1.000 0、0.999 9、1.000 0;平均加样回收率（n=9）分别为100.22%、100.62%、100.02%、100.33%,且RSD分别为0.99%、1.18%、2.02%、0.97%。结论：该方法简便可行、准确、可靠,重现性好,结果稳定,可为血塞通软胶囊的质量控制方法提供参考。

Objective： To establish the method for determining the notoginsenoside R1, ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Xuesaitong soft capsules by HPLC. Methods： The separation was performed on ZORBAX SB-Aq column（250 mm × 4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile-water with linear gradient elution. The flow rate was 1.0 m L·min-1. The detection wavelength was 203 nm and the column temperature was 25 ℃. Results： The calibration curves showed good linearity in the range of 0.32 – 3.20 μg（notoginsenoside R1, r = 1.000 0）, 0.72 – 7.20 μg（ginsenoside Rg1, r = 1.000 0）, 0.21 – 2.10 μg（ginsenoside Re, r = 0.999 9）, 0.92 – 9.20 μg（ginsenoside Rb1, r = 1.000 0）. The average recoveries（n = 9） were 100.22%, 100.62%, 100.02%, 100.33% respectively, and RSD were 0.99%, 1.18%, 2.02%, 0.97% respectively. Conclusion： The method is convenient, rapid, economical and accurate, which is suitable for quality control of Xuesaitong soft capsules.