Caspase-3、TNF-α、bcl-2、bax在人胎儿卵巢中的表达

Expression of the Caspase-3,TNF-α,bcl-2 and bax in the fetal ovarian

目的 探讨人胎儿卵泡的发育形成过程中卵细胞凋亡及其调控机制。方法 收集2013年1-12月广东省计划生育科学技术研究所妊娠24-32周引产的女性胎儿卵巢组织18例,其中24-27^＋6周胎儿卵巢8例,28-32周胎儿卵巢10例。采用免疫组织化学染色法观察胎儿卵巢卵泡发育及形成过程中凋亡因子Caspase-3、TNF-α、bcl-2、bax的表达情况。结果 Caspase-3表达在孕中期及孕晚期表达均为强阳性,TNF-α在卵母细胞中均表达,胎儿卵巢内的颗粒细胞及其他间质组织细胞未见阳性表达。凋亡抑制因子bcl-2的表达在孕中期及孕晚期均为阴性。促凋亡因子Bax在卵母细胞及卵巢颗粒细胞内均为阳性表达。结论 胎儿卵巢发育过程中,原始卵母细胞不断发生凋亡,由四种凋亡因子同时共同参与,而bcl-2和bax可能通过活化Caspase-3引起少量颗粒细胞凋亡。

Objective To investigate the apoptosis and the expression of apoptosis related protein Caspase-3,TNF-α,bcl-2,bax of the oocyte during human fetal development. Methods Ovarian tissues from 18 fetuses（aged from 24 to32 weeks） were obtained after labortion induced by mifepristone and PG from January to December 2013 in Guangdong Family Planning Research Institute,including 8 fetuses of midpregnancy（aged 24-27^＋6weeks） and 10 fetuses of latepregnancy（aged 28-32 weeks）. The morphology and development of oocyte were observed using HE-staining and immunohistochemistry. The expression of the apoptosis related protein,such as Caspase-3,TNF-α,bcl-2,bax of the oocyte during human fetal follicular development were examined. Results The Caspase-3 was positive both in the fetal oocytes and the granulosa cells. TNF-α was positive in the oocytes. Bcl-2 was negative both in the fetal oocytes and the granulosa cells. Bax was positive both in the fetal oocytes and the granulosa cells. Conclusion The four apoptosis factors are all participate in the oocyte apoptosis during human fetal development. bcl-2 and bax may result in the granulosa cells apoptosis participate through activate the Caspase-3.