桑菊口服液抗菌活性成分研究

Study on antibacterial constituents of Sangju oral liquid

目的 对桑菊口服液抗菌活性成分进行研究。方法 采用反相硅胶柱层析分离技术对桑菊口服液进行有效部位的分离,在体外抗菌活性筛选结果的指导下利用现代色谱分离方法（硅胶柱色谱、羟丙基葡聚糖凝胶色谱以及制备液相等）分离有效部位各成分化合物,通过理化常数测定和光谱分析鉴定其化学结构。结果 从桑菊口服液抗菌活性部位分离鉴定了9个酚酸类化学成分：3-（3-羟基-苯基）-丙酸、3-（3-羟基-苯基）-丙酸甲酯、肉桂酸、3-羟基-1-（3-甲氧基-4-羟基苯）丙烷-1、3-羟基-1-（3,5-二甲氧基-4-羟基苯）丙烷-1、对羟基苯甲酸、原儿茶酸、香草酸、没食子酸。结论 桑菊口服液的抗菌作用源于酚酸类化学成分的作用,且化合物1-9为首次从桑菊口服液中分离得到。

Objective To study antibacterial constituents of Sangju Oral Liquid. Methods The effective parts of Sangju Oral Liquid were isolated by reversal phase silica gelcolumn chromatography,under the guidance of the screening antibacterial activity results in vitro,the compounds of Sangju Oral Liquid effective parts were isolated by modern chromatographic separation（silica gelcolumn chromatography,sephhadex LH-20,Prep-HPLC and so on）. On the basis of spectroscopic methods and physicochemical properties,the structures of these compounds were elucidated. Results Nine constituents were isolated and elucidated from antibacterial active site of Sangju Oral Liquid,the structures of 9compounds were： 3-（3-hydroxyl-phenyl）- propionate,3-（3-hydroxyl-phenyl）- methoxypropionate,cinnamic acid,3-hydroxy-1-（3-methoxy-4-Hydroxy phenyl） propan-1-one,3-hydroxy-1-（3,5-dimethoxy-4-Hydroxyphenyl）propan-1-one,p-hydroxybenzoic acid,protocatechuic acid,vanilloid,gallic acid. Conclusion The antibacterial activity of Sangju Oral Liquid stems from phenolicacid constituents,and compounds 1-9 are the first time isolated from Sangju Oral Liquid.