肥胖及糖尿病食蟹猴全天血糖、胰岛素值及相关生理指标观测

Continuous Monitoring on Blood Glucose and Insulin Levels in Obesity and Diabetes Cynomolgus Monkeys

目的探讨肥胖及糖尿病食蟹猴全天24h血糖、胰岛素变化情况及相关生理指标。方法选择36只成年雄性食蟹猴，其中正常、肥胖和糖尿病食蟹猴各12只。测量每只食蟹猴体质量、腹围、皮脂，计算体质量指数（BMI）；并检测各食蟹猴心率、血压、呼吸及体温；在24h内，每1．5h进行一次采血，检测血糖、胰岛素、糖化血红蛋白及血脂四项。结果正常、肥胖和糖尿病食蟹猴的血糖值分别为3．94±0．65mmol／L、4．33±0．84mmol／L和12．09±4．58mmol／L；糖尿病食蟹猴在21：00～22：30时间段血糖值最高为13．68±0．55mmol／L，18：00-19：30血糖值最低为10．94±0．49mmol／L。正常、肥胖和糖尿病食蟹猴24h胰岛素值为（29．50±12．36）mU／L-（62．50±21．98）mU／L、（31．21±0．98）mU／L-（123．42±3．38）mU／L和（12．60±3．16）mU／L-（30．62±3．16）mU／L；糖尿病食蟹猴9：00～10：30胰岛素值最大，为30．62±11．71mU／L，03：00-04：30胰岛素值最低，为12．60±3．16mu／L。结论食蟹猴基础血糖值和胰岛素值的调查为糖尿病动物模型的建立及其相关研究提供基础数据参考。

Objective To explore the dynamic changes of the glucose and insulin levels of obesity and diabetes cynomolgus monkey during 24 hours Cycle. Methods Blood glucose and insulin levels from 36 adult male cynomolgus monkeys, which were uniformly selected from obesity, diabetes and normal group, were examined. Parameters including body weight, abdominal circumference, skin fold thickness, calculated body mass index （BMI）, heart rate, blood pressure, breathing and body temperature were detected. Glucose, insulin, glycosylated hemoglobin and lipids levels were recorded by a interval of 1.5 hours in all individuals. Results The blood glucose value in normal, obesity and diabetes cynomol- gus monkeys were 3.94 ± 0.65 mmol/L, 4.33 ± 0.84 mmol/L, and 12.09 ± 4.58 mmol/L respectively. The blood glucose level of diabetes cynomolgus monkeys reached the peak during 21 ： 0022 ： 30 with 13.68 ± 0.55 mmol/L, and reached lowest point during 18：00-19：30 with 10.94± 0.49 mmol/L. The insulin values in normal, obesity and diabetes cynomolgus monkeys were （29.50 ± 12.36）-（62.50 ± 21.98） mU/L, （31.21 ± 0.98）-（123.42 ± 3.38）mU/L, and （12.60 ± 3.16）-（30.62 ± 3.16）mU/L respectively. The maximum insulin value of diabetes monkeys was 30.62 ± 11.71 mU/L during 9 ： 00-10 ： 30, and minimum of 12.60 ± 3.16 mU/L during 03 ： 00-04 ： 30. Conclusion This study provides an immediate quantitative basis for investigating of glucose and insulin levels in cynomolgus monkeys, and it sets up a fundamental reference for primate models of diabetes or related diseases.