当归芍药散对慢性盆腔炎模型大鼠分子免疫调控的影响

Effect of Danggui Shaoyao Powder on Molecular Immune Regulation of Chronic Pelvic Inflammatory Disease Model Rats

目的:明确当归芍药散抗炎抗粘连的药理作用,探讨当归芍药散治疗慢性盆腔炎的免疫学机制。方法:采用混合细菌加机械损伤造模法制备慢性盆腔炎大鼠模型,测定ICAM-1、SIg-A、TNF-α和IL-1的阳性表达水平和SIg-A浓度变化。结果:给药组中、高剂量组大鼠ICAM-1、SIg-A、TNF-α和IL-1的检测值与模型组比较有明显差异(P<0.05),其中高剂量组与模型组比较有极显著差异(P<0.01),高剂量组与对照组中SIg-A浓度比较有显著差异(P<0.05)。结论:证实了当归芍药散通过调节机体免疫功能治疗慢性盆腔炎,明确了当归芍药散抗炎抗粘连及改善免疫功能的作用机制。

Objective： To clear pharmacological effects of Danggui Shaoyao Powder anti- inflammatory and anti- adhesion and to study immunological mechanisms of Danggui Shaoyao Powder on chronic pelvic inflammatory disease. Methods：The rats were administered with mixed bacteria and mechanical damage to establish chronic pelvic inflammatory disease model and to detect the positive expression levels of ICAM- 1,SIg- A,TNF- α and IL- 1 and concentration changes of SIg- A. Results： The expressions of ICAM- 1,SIg- A,TNF- α and IL- 1 had obvious difference between middle and high dose groups and model group（ P 〈0. 05）,especially the high dose group（ P〈 0. 01）. The expression of SIg- A had significant difference between high dose group and control group（ P〈 0. 05）. Conclusion： Danggui Shaoyao Powder can treat chronic pelvic inflammatory disease by regulating immune function. This study clarified the mechanism of Danggui Shaoyao Powder anti- inflammatory and anti- adhesion and improving immune function.