摘要
目的:观察加味聚精食疗方对少、弱精子症小鼠睾丸组织中CR16表达的影响。方法:60只8周龄雄性小鼠适应性饲养1周后随机编号,采取区组随机化法将小鼠分为6组:正常组(G1)、DS模型空白对照组(G2)、低剂量治疗组(G3)、中剂量治疗组(G4)、高剂量治疗组(G5)、预防治疗组(G6),每组10只。G2~G6组通过尾静脉单剂量注射5-FU(100 mg/kg),14 d后形成稳定的DS模型。G1正常组全程给予蒸馏水4 g/kg灌胃,连续40 d;G2空白对照组及G3~G5治疗组自造模开始即给予蒸馏水4 g/kg灌胃,G6预防治疗组给予加味聚精食疗方6.3 g/kg灌胃,连续14 d。G2~G6组造模完成后,G2组予蒸馏水4 g/kg灌胃;G3~G5组分别予加味聚精食疗方低剂量(3.15 g/kg)灌胃、中剂量(6.3 g/kg)灌胃、高剂量(12.6 g/kg)灌胃;G6组继续蒸馏水4 g/kg连续灌胃。以上均1次/d,连续喂养26 d。各组治疗结束后作集中处死,计算机辅助精子分析(CASA)技术检测小鼠附睾精子质量;对小鼠肝、肾器官及睾丸组织进行病理学检查;免疫组织化学S-P法检测基因CR16在SD动物模型体内的表达。结果:在精子质量的改善方面,G2、G6组比较无明显差异,不具有统计学意义(P〉0.05)。G3、G4、G5组均较G2、G6组有明显改善(P〈0.05),且G4组优于G3、G5组(P〈0.05)。在阴性对照无特异性着色的情况下对结果进行判读,G3、G4、G5组小鼠睾丸组织生精小管上皮中CR16均有不同程度的表达,明显高于G2、G6组,差异具有统计学意义(P〉0.05),G4组小鼠睾丸组织结构修复情况、睾丸组织CR16的表达水平(染色程度)均明显上调。镜下观察见小鼠睾丸组织中CR16阳性反应呈棕褐色或浅棕色颗粒状,散在分布于支持细胞胞浆胞膜。结论:加味聚精食疗方能够一定程度上提高附睾精子的质量,安全无不良反应,其通过上调DS动物模型体内CR16的表达水平可能是治疗少、弱精子症的作用机制之一。
Objective: To observe the influence of Jiawei Jujing Shiliao Prescription on the CR16 expression in oligozoospermia spermatozoa rats. Methods: Sixty rats were randomized into six groups,with ten rats in each group. Among them,rats in Group 2 to Group 6 were administered with 5- FU iv( 100 mg / kg) for fourteen days to establish the DS models. Rats in Group 1 were given distilled water( 4 g / kg) for continuous 40 days. After model establishment,rats in Group 2 and Group 6 were intragastrically administered with distilled water( 4 g / kg) and rats in Group 3 to Group 5 were intragastrically administered with Jujing Shiliao Prescription with low dose( 3. 15 g / kg),moderate dose( 6. 3 g / kg) and high dose( 12. 6 g / kg) respectively,for 26 days,and in Group 6,before model establishment,Jujing Shiliao Prescription( 6. 3 g/kg) was applied for 14 days. All the rats were sacrificed after treatments. The sperm quality in the epididymis was detected with computer- assisted sperm analysis( CASA) technique and the pathological changes of the liver,kidney and testes were observed. The CR16 expression in the rats was explored with immunohistochemical method. Result: Forthe sperm quality,there was no significant difference between Group 2 and Group 6( P 〉0. 05). Compared with those in Group 2 and Group 6,there was significant improvement in Group 3,4 and 5( P〈 0. 05),with Group 4 better than Group3 and Group 5( P 〈0. 05). The CR 16 expression in Group 3,4 and 5 was apparently higher than those in Group 2 and 6( P 〈0. 05). Conclusions: Jiawei Jujing Shiliao Prescription could improve the sperm quality in the epididymis. Meanwhile,it could up- regulate the expression of CR16,which may be one of the mechanisms for the treatments of oligozoospermia spermatozoa.
出处
《中华中医药学刊》
CAS
北大核心
2015年第11期2690-2693,共4页
Chinese Archives of Traditional Chinese Medicine
基金
广东省中医药局科研项目(20131042)
关键词
加味聚精食疗方
精子质量
CR16
Jiawei Jujing Shiliao Prescription
sperm quality
CR16