摘要
目的:研究石杉碱甲(Hup-A)大鼠在体肠吸收部位。方法:运用大鼠在体低速单向肠灌流模型,以酚红为标示物,分别研究了不同浓度及不同肠段Hup-A的吸收情况。通过肠腔有效吸收系数(Peff)、吸收速率常数(Ka)和药物吸收剂量分数(fa)评价药物的吸收部位,考察Hup-A的吸收动力学。结果:Hup-A在0.5-10.0μg·m L^-1浓度内,药物累积吸收量随着灌流液药物浓度的增加而提高,Hup-A的吸收曲线近似线性状态,无浓度抑制作用,且不同浓度Hup-A的Peff,Ka,fa均无显著性差异(P〉0.05)。当灌流液浓度为10μg·m L^-1时,Hup-A在大鼠各肠段的Peff、Ka和fa依次为十二指肠〉回肠〉空肠〉结肠,且结肠与其他肠段均有显著性差异(P〈0.05),其Peff值分别为(0.57±0.04)×10^-4cm^-1·s^-1,(0.53±0.02)×10^-4cm^-1·s^-1,(0.48±0.05)×10^-4cm^-1·s^-1,(0.41±0.06)×10^-4cm^-1·s^-1。结论:Hup-A在大鼠各肠段均有不同程度的吸收,药物浓度对Hup-A的肠吸收无影响。因此,其在肠道的吸收呈现一级吸收动力学,吸收机制为被动扩散。
Objective: To investigate the Huperize A absorption in parts of rats intestine. Method: After low- speed single pass perfusion model of rats in situ was set up,by takingphenolsulfonphthalein as a marker,the absorption kinetics of Huperize A in small intestine was explored under conditions of differentconcentrationsand segments. The drugabsorption was evaluated by effective permeability( Peff),absorption rate constant( Ka) and fraction absorbed( fa). Results: With the increase of Huperize A's concentration range of 0. 5- 10. 0 μg·m L^- 1,the accumulative absorption of Huperize A was increased and its absorption curve was to be linear,with no inhibition due to differentconcentrations. There was no significant difference of absorption parameters of Peff,Kaand faunder conditions of different concentrations( P 〉0. 05). The Peff,Kaand faof Huperize A at concentration of 10. 0 μg. m L^- 1in four different regions of rats intestine were in the following sequence: duodenum〉 ileum〉 jejunum 〉colon and the Peff of Huperize Awas( 0. 57 ± 0. 04) × 10^- 4·cm^- 1·s^- 1,( 0. 53 ± 0. 02) × 10^- 4·cm^- 1·s^- 1,( 0. 48 ± 0. 05) × 10^- 4·cm^- 1·s^- 1,( 0. 41 ± 0. 06) × 10^- 4·cm^- 1·s^- 1. Conclusions: Huperize A could be absorbed at varioussegments of intestinal wall. The absorption of Hup- A appeared as a first- order process with a passive diffusion mechanism.
出处
《中华中医药学刊》
CAS
北大核心
2015年第11期2721-2724,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省新世纪151人才工程(第二层次)
浙江省医学重点学科群建设项目(XKQ-010-001)
关键词
在体单向肠灌流
石杉碱甲
吸收部位
吸收机制
Huperize A
in situ single pass perfusion model
absorption site
bsorption mechanism