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Orexin-A受体介导的生长抑素激动剂ODT8-SST对大鼠摄食和饮水的影响

Effect of Orexin-A Receptor Mediated Somatostatin Agonists ODT8-SST of Rats on Food Intake and Water Intake
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摘要 目的:探讨orexin-A(OXA)受体介导的生长抑素激动剂ODT8-SST对大鼠摄食和饮水的调节作用相关作用机制。方法:在光照周期内,大鼠40只随机分8组,侧脑室(icv)分别注射不同剂量ODT8-SST或生理盐水(NS);大鼠56只随机分8组分别侧脑室注射不同剂量OXA受体(OX1R)拮抗剂SB-334867或NS;2小时后测量大鼠摄食量和饮水量。结果:与NS组相比,实验组大鼠侧脑室注射ODT8-SST(1μg/rat),2小时后摄食量和饮水量均显著增加(P<0.05)。大鼠侧脑室注射SB-334867(16μg/rat)完全抑制了由侧脑室注射ODT8-SST后引起的摄食量和饮水量的增加;与此相反,大鼠给予SST2拮抗剂S-406-028预处理之后,可阻止侧脑室注射ODT8-SST引发的促进食欲作用,但不会影响侧脑室注射OXA(10.7μg/rat)诱导的摄食量和饮水量的增加。结论:侧脑室注射ODT8-SST可促进摄食和饮水,该过程可能由OX1R所介导;orexin-A促进摄食作用不依赖大脑SST2通路的激活。 Objective： To investigate the orexin-A（OXA） receptor mediated somatostatin agonists ODT8-SST on the regulation of food intake and water intake of rats and OXA promoting feeding mechanism. Methods： In the light period, 40 rats were randomly divided into 8 groups, the lateral ventricle（icv） were injected with different doses of ODT8-SST or normal saline（NS）; 56 rats were randomly divided into 8 groups, icv were injected with different doses of OXA receptor（OX1R） antagonist SB-334867 or NS; the food intake and water intake of rat were recorded for 2 hours after the injection. Results： Compared with NS control group, after injecting ODT8-SST（1μg / rat, icv） into ICV, the food intake and water intake of experimental group were significantly increased（P 0.05）. Lateral ventricle injection of OXA receptor（OX1R） antagonist SB-334867（16 μg / rat） completely inhibited the increase in food intake and water intake caused by lateral ventricle injection of ODT8-SST; with in contrast, rats were given SST2 antagonist S-406-028 after pretreatment, can prevent the promotion of the role of appetite caused by intracerebroventricular injection of ODT8-SST, but does not affect the lateral ventricle injection OXA（10.7 μg/rat） induced food intake and water intake increased. Conclusions： Intracerebroventricular injection of ODT8-SST can promote feeding and drinking, OX1 R signaling pathway is a part of the neural circuits; orexin-A promotes the ingestion does not depend on the activation of the brain SST2 pathway.
作者 黄晓晖 彭晓燕 逄明杰 郭菲菲 孙向荣 公衍玲 徐珞
机构地区 青岛大学医学院病理生理教研室 菏泽市传染病医院 青岛市立医院 青岛科技大学
出处 《现代生物医学进展》 CAS 2015年第28期5424-5427,共4页 Progress in Modern Biomedicine
基金 国家自然科学基金项目(81470815 31071014 81100260 81270460 81300281) 山东省优秀中青年科学家科研奖励基金项目(BS2014YY009) 青岛市科技局项目(13-1-4-170-jch 14-2-3-3-nsh)
关键词 摄食量 食欲素A ODT8-SST SB-334867 Food intake Orexin-A ODT8-SST SB-334867
分类号 Q95-3 [生物学—动物学] R338.2 [医药卫生—人体生理学]
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参考文献25

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现代生物医学进展
2015年 第28期

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