根皮素对HepG2肝癌细胞增殖和凋亡影响的实验研究

Effect of Phloretinon Proliferation and Apoptosis of HepG2 Cells

目的:探讨根皮素对人肝癌Hep G2细胞增殖和凋亡的影响。方法:MTT法检测不同浓度(30、40、50μg/m L)根皮素对肝癌Hep G2细胞增殖的抑制作用,流式细胞技术检测根皮素对Hep G2细胞凋亡的影响,ELISA法检测不同浓度根皮素干预后细胞中Bcl-2和Bax表达的变化,Western blot法检测30μg/m L根皮素在8,16,24小时后检测p-AKT蛋白表达情况。结果:30、40和50μg/m L的根皮素对肝癌Hep G2细胞的增殖均有抑制作用(P<0.01),同时,30、40和50μg/m L的根皮素在24小时后可诱导Hep G2细胞发生早期凋亡,凋亡率分别为0.1321±0.0224,0.2607±0.0457,0.3712±0.0884(P<0.01);另外,30、40和50μg/m L的根皮素作用24小时后细胞内Bcl-2表达降低,Bax表达增高(P<0.01);最后,30μg/m L根皮素可以明显减少p-AKT表达量,这种作用呈现时间依赖性。结论:根皮素能够抑制肝癌Hep G2细胞的增殖和促进细胞凋亡。

Objective： To evaluate the effects of phloretin on proliferation and apoptosis of Hep G2 cells. Methods： Effects of phloretin on Hep G2 cell proliferation and apoptosis were evaluated by MTT method and flow cytometry instrument, respectively.Expression of Bcl-2 and Bax protein in Hep G2 cell with or without phloretin pretreatment was detected by ELISA method. Expression of the p-AKT protein in Hep G2 cell which were treated with phloretin（30 μg/m L） in 8, 16, 24 hours can be measured by Western Blot.Results： Different doses of phloretin（30, 40 and 50 μg/m L） depressed the proliferation of Hep G2 cells（P〈0.01）. Besides, all dosages of phloretin（30, 40 and 50 μg/m L） induced cell apoptosis in Hep G2 significantly compared to that of control,the relative rate of apoptosis：0.1321±0.0224, 0.2607±0.0457, 0.3712±0.0884（P〈0.01）. What was more, 30, 40 and 50 μg/m L phloretin decrease the expression of Bcl-2 and increased Bax expression, respectively; and phloretin can decrease the expression of the p-AKT. Conclusion： Phloretin could inhibit proliferation and induced apoptosis on Hep G2 cells.