高胆固醇饮食的兔丁酰胆碱酯酶活性与血脂的相关性分析

Correlative Analyses between Butyrylcholinesterase Activity and Blood Lipid in High Cholesterol Diet Rabbit

目的:通过建立高血脂动物模型,探索高血脂动物血浆丁酰胆碱酯酶(Bu Ch E)的活性与血脂指标的相关性,为Bu Ch E活性作为高血脂疾病标志物的可能性提供参考。方法:雄性新西兰兔14只,随机分为两组(每组各7只),即普通饲料组(C组)和高胆固醇饲料组(H组)。连续喂养12周,每二至三周称重采血,采用Ellman法检测Bu Ch E活性,按照试剂盒使用说明测定血浆总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C);12周后,麻醉兔子开胸采集胸主动脉,经10%的中性福尔马林固定后进行苏丹Ⅳ脂肪染色。结果:兔子喂食高胆固醇饲料12周后,H组动脉出现斑块,C组无斑块形成;兔子在食用高胆固醇饲料的过程中,血浆TC、LDL-C、HDL-C显著升高(P<0.01),而Bu Ch E活性无显著变化(P>0.05);分析正常兔子和高血脂兔子血浆Bu Ch E活性与TC、TG、HDL-C、LDL-C的皮尔森相关系数,发现均无相关性(r<<1,P>0.05)。结论:本项研究未在正常兔子和高血脂的兔子中发现Bu Ch E与TC、TG、HDL-C、LDL-C存在相关性,说明通过喂食兔子高胆固醇饲料建立的高血脂模型不适合探究Bu Ch E与TC、TG、HDL-C、LDL-C的相关性。

Objective： To compare the correlation between butyrylcholinesterase（Bu Ch E） activity and blood lipid in normal and hyperlipidemia rabbit and to explore the potential of using Bu Ch E as a biomarker for hyperlipidemia. Methods： 14 male New Zealand rabbits were randomly divided into two groups（7 rabbits each）, normal diet group（group C） and high-cholesterol diet group（group H）.All rabbits were fed with either normal diet or high-cholesterol diet for 12 weeks. The body weight of each rabbit was recorded and the peripheral blood was collected every 2 to 3 weeks. The activity of Bu Ch E was detected with Ellman＇s assay and the concentration of plasma total cholesterol（TC）, triglyceride（TG）, high-density lipoprotein cholesterol（HDL-C） and low-density lipoprotein cholesterol（LDL-C） was analyzed with commercial kits. After 12 weeks, rabbits were anaesthetized with urethane, the aorta thoracicas were taken out and fixed in 10 % neutral formaldehyde for 24 hours, then stained with Sudan Ⅳ. Results： After 12 weeks, clear atherosclerotic plaques were found in the aorta thoracicas of high-cholesterol diet group（Group H） but not in that of normal diet group（Group C）. With the progress of high cholesterol diet, the TC, LDL-C and HDL-C level in the plasma of group H was much higher compare to that in group C（P〈0.01）, while Bu Ch E activity remained the same compare to the 0 week. The results of the Pearson correlation indicated that there was no significant correlation between Bu Ch E activity and TC, TG, HDL-C, LDL-C level（r〈〈1, P〉0.05）. Conclusions： The result of this research showed that there was no significant relationship between Bu Ch E activity and TC, TG, HDL-C, LDL-C level, which suggests that the cholesterol-fed hyperlipidemia rabbit model is not suitable for the correlation analyses between Bu Ch E activity and TC, TG,HDL-C and LDL-C.